Type

Journal Article

Authors

David G Little
Lauren Peacock
Kathy Mikulec
Laurence C Cantrill
Nicole YC Yu
John P Gleeson
Aaron Schindeler
Fergal O'Brien
Ciara M Murphy

Subjects

Biochemistry

Topics
rats wistar recombinant proteins durapatite x ray microtomography bone and bones collagen collagen binding male osteoclasts tissue engineering cell death porosity anatomy fluorescent dyes drug delivery systems zoledronic acid binding proteins osteogenesis animals rats isoenzymes acid phosphatase prosthesis implantation tissue scaffolds wistar bone morphogenetic proteins collagen scaffolds mice humans diphosphonates bisphosphonate

A collagen hydroxyapatite scaffold allows for binding and co-delivery of recombinant bone morphogenetic proteins and bisphosphonates (2014)

Abstract An emerging paradigm in orthopedics is that a bone-healing outcome is the product of the anabolic (bone-forming) and catabolic (bone-resorbing) outcomes. Recently, surgical and tissue engineering strat- egies have emerged that combine recombinant human bone morphogenetic proteins (rhBMPs) and bis- phosphonates (BPs) in order to maximize anabolism and minimize catabolism. Collagen-based scaffolds that are the current surgical standard can bind rhBMPs, but not BPs. We hypothesized that a biomimetic collagen?hydroxyapatite (CHA) scaffold would bind both agents and produce superior in vivo outcomes. Consistent with this concept, in vitro elution studies utilizing rhBMP-2 ELISA assays and scintillation counting of 14C-radiolabeled zoledronic acid (ZA) confirmed delayed release of both agents from the CHA scaffold. Next, scaffolds were tested for their capacity to form ectopic bone after surgical implanta- tion into the rat hind limb. Using CHA, a significant 6-fold increase in bone volume was seen in rhBMP-2/ ZA groups compared to rhBMP-2 alone, confirming the ability of ZA to enhance rhBMP-2 bone formation. CHA scaffolds were found to be capable of generating mineralized tissue in the absence of rhBMP-2. This study has implications for future clinical treatments of critical bone defects. It demonstrates the relative advantages of co-delivering anabolic and anti-catabolic agents using a multicomponent scaffold system. Crown
Collections Ireland -> Trinity College Dublin -> Administrative Staff Authors (Scholarly Publications)
Ireland -> Trinity College Dublin -> Administrative Staff Authors
Ireland -> Royal College of Surgeons in Ireland -> Anatomy Articles
Ireland -> Royal College of Surgeons in Ireland -> Department of Anatomy

Full list of authors on original publication

David G Little, Lauren Peacock, Kathy Mikulec, Laurence C Cantrill, Nicole YC Yu, John P Gleeson, Aaron Schindeler, Fergal O'Brien, Ciara M Murphy

Experts in our system

1
John P Gleeson
Royal College of Surgeons in Ireland
Total Publications: 26
 
2
Aaron Schindeler
Royal College of Surgeons in Ireland
Total Publications: 4
 
3
Fergal J O'Brien
Royal College of Surgeons in Ireland
Total Publications: 268
 
4
Ciara M Murphy
Royal College of Surgeons in Ireland
Total Publications: 16