Type

Journal Article

Authors

John P Gleeson
Glenn R Dickson
Amos Matsiko
Fergal O'Brien
Tanya J Levingstone

Subjects

Biochemistry

Topics
collagen type ii osteochondral defect repair osteochondral defects tissue engineering material properties cartilage tissue engineering multi layered scaffolds bone marrow anatomy novel the collagen

A biomimetic multi-layered collagen-based scaffold for osteochondral repair (2014)

Abstract Cartilage and osteochondral defects pose a significant challenge in orthopedics. Tissue engineering has shown promise as a potential method for the treatment of such defects; however, a long-lasting repair strategy has yet to be realized. This study focuses on the development of a layered construct for osteo- chondral repair, fabricated through a novel ``iterative layering?? freeze-drying technique. The process involved repeated steps of layer addition followed by freeze-drying, enabling control over material composition, pore size and substrate stiffness in each region of the construct, while also achieving a seamlessly integrated layer structure. The novel construct developed mimics the inherent gradient struc- ture of healthy osteochondral tissue: a bone layer composed of type I collagen and hydroxyapatite (HA), an intermediate layer composed of type I collagen, type II collagen and HA and a cartilaginous region composed of type I collagen, type II collagen and hyaluronic acid. The material properties were designed to provide the biological cues required to encourage infiltration of host cells from the bone marrow while the biomechanical properties were designed to provide an environment optimized to promote differen- tiation of these cells towards the required lineage in each region. This novel osteochondral graft was shown to have a seamlessly integrated layer structure, high levels of porosity (>97%), a homogeneous pore structure and a high degree of pore interconnectivity. Moreover, homogeneous cellular distribution throughout the entire construct was evident following in vitro culture, demonstrating the potential of this multi-layered scaffold as an advanced strategy for osteochondral defect repair.
Collections Ireland -> Trinity College Dublin -> Administrative Staff Authors (Scholarly Publications)
Ireland -> Trinity College Dublin -> Administrative Staff Authors
Ireland -> Royal College of Surgeons in Ireland -> Anatomy Articles
Ireland -> Royal College of Surgeons in Ireland -> Department of Anatomy

Full list of authors on original publication

John P Gleeson, Glenn R Dickson, Amos Matsiko, Fergal O'Brien, Tanya J Levingstone

Experts in our system

1
John P Gleeson
Royal College of Surgeons in Ireland
Total Publications: 26
 
2
Glenn R Dickson
Royal College of Surgeons in Ireland
Total Publications: 7
 
3
Amos Matsiko
Royal College of Surgeons in Ireland
Total Publications: 24
 
4
Fergal J O'Brien
Royal College of Surgeons in Ireland
Total Publications: 265
 
5
Tanya J Levingstone
Royal College of Surgeons in Ireland
Total Publications: 28