Journal Article


Michael D Welsh
Simon Doherty
Colin Foster
Bryan Markey
Joseph P. Cassidy
Bernadette Earley
Fawad Mansoor



administration dosage nanoparticles polylactic acid polyglycolic acid copolymer pharmacology viral vaccines animals respirovirus infections disease models animal veterinary immunity humoral standards mice virology cattle diseases polyglycolic acid female prevention control antibodies viral cattle immunology enzyme linked immunosorbent assay blood administration intranasal mice inbred balb c lactic acid random allocation parainfluenza virus 3 bovine

Intranasal delivery of nanoparticles encapsulating BPI3V proteins induces an early humoral immune response in mice. (2013)

Abstract Vaccine adjuvants are typically designed to stimulate both systemic and mucosal immune responses. Polymeric nanoparticles have been used as adjuvants in the development of vaccines against a number of viral pathogens and tested in laboratory animals. The objective of the study was to assess if synthetic bovine parainfluenza virus type-3 (BPI3V) peptide motifs and solubilised BPI3V proteins encapsulated in poly (dl-lactic-co-glycolide) (PLGA) nanoparticles (NPs) induce specific humoral immune responses in a mouse model following intranasal administration. BPI3V-specific and peptide specific IgG ELISAs were used to measure serum IgG levels to BPI3V. Intranasal delivery of PLGA nanoparticles encapsulating BPI3V proteins elicited an early, gradually increasing BPI3V-specific IgG response that persisted over the subsequent 6 weeks, suggesting slow, persistent release of antigen. PLGA-BPI3V particles administered intranasally induced a stronger IgG antibody response at an earlier time point compared with solubilised BPI3V antigen alone. Such an approach could be deployed in the development of new generation vaccines.
Collections Ireland -> Teagasc -> PubMed

Full list of authors on original publication

Michael D Welsh, Simon Doherty, Colin Foster, Bryan Markey, Joseph P. Cassidy, Bernadette Earley, Fawad Mansoor

Experts in our system

Michael D Welsh
Total Publications: 11
B K Markey
University College Dublin
Total Publications: 43
Joseph P. Cassidy
University College Dublin
Total Publications: 55
Bernadette Earley
Total Publications: 120