Type

Journal Article

Authors

Colin Hill
R Paul Ross
Barry Kiely
Fergus Shanahan
Maria Walsh
Peadar G Lawlor
Paula M. O'Connor
Pat G. Casey
Debebe Alemayehu
Mary C. Rea

Subjects

Microbiology

Topics
bacteriocins bacterial shedding thuricin administration rectal feces drug effects swine chemistry clostridium difficile administration oral animals microbiology gastrointestinal tract bacillus thuringiensis metabolism anti bacterial agents biological availability mice pharmacokinetics isolation purification growth development administration dosage

Bioavailability of the anti-clostridial bacteriocin thuricin CD in gastrointestinal tract. (2013)

Abstract Thuricin CD is a two component narrow spectrum bacteriocin comprising two peptides with targeted activity against Clostridium difficile. This study examined the bioavailability of thuricin with a view to developing it as an effective antimicrobial against intestinal infection. One of the peptides, Trn-β, was found to be degraded by the gastric enzymes pepsin and α-chymotrypsin both in vitro and in vivo, whereas Trn-α was resistant to digestion by these enzymes and hence was detected in the intestinal porcine digesta following oral ingestion by pigs. In order to determine if spores of the producing organism Bacillus thuringiensis DPC 6431 could be used to deliver the bacteriocin to the gut, spores were fed to 30 mice (approx. 10(8)-2×10(8) per animal) and their germination, growth and production of thuricin in the gastrointestinal tract (GIT) of the animals was monitored. Almost 99 % of the spores delivered to the GIT were excreted in the first 24 h and neither Trn-α nor Trn-β was detected in the gut or faecal samples of the test mice, indicating that ingestion of B. thuringiensis spores may not be a suitable vehicle for the delivery of thuricin CD. When thuricin CD was delivered rectally to mice (n = 40) and C. difficile shedding monitored at 1, 6, 12 and 24 h post-treatment, there was a >95 % (>1.5 log units) reduction of C. difficile 027 in the colon contents of infected mice (n = 10) 1 h post-treatment compared with the control group (n = 10; P<0.001). Furthermore, 6 h post-treatment there was a further 1.5 log reduction in C. difficile numbers (n = 10) relative to the control group (n = 10; P<0.05). These results would suggest that rectal administration of thuricin may be a promising mode of delivery of thuricin CD to the colon.
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Full list of authors on original publication

Colin Hill, R Paul Ross, Barry Kiely, Fergus Shanahan, Maria Walsh, Peadar G Lawlor, Paula M. O'Connor, Pat G. Casey, Debebe Alemayehu, Mary C. Rea

Experts in our system

1
Colin Hill
University College Cork
Total Publications: 351
 
2
R Paul Ross
Teagasc
Total Publications: 441
 
3
Barry Kiely
University College Cork
Total Publications: 21
 
4
Fergus Shanahan
University College Cork
Total Publications: 237
 
5
Maria C. Walsh
Teagasc
Total Publications: 16
 
7
Paula M. O'Connor
Teagasc
Total Publications: 85
 
8
Pat G. Casey
University College Cork
Total Publications: 40
 
9
Mary C. Rea
Teagasc
Total Publications: 68