Type

Journal Article

Authors

M A Crowe
A. C. Evans
M. G. Diskin
M. Hilliard
G. Elia
M. P. Mullen
M E Beltman

Subjects

Biochemistry

Topics
pre implantation embryo implantation platelet activating factor beef quality 1 protein embryonic development mass spectrometry global

Global proteomic characterization of uterine histotroph recovered from beef heifers yielding good quality and degenerate day 7 embryos. (2013)

Abstract The objective was to analyze the proteomic composition of uterine flushes collected from beef heifers on day 7 after insemination. Estrus was synchronized in crossbred beef heifers by using a protocol with a controlled intravaginal drug releasing device. Heifers detected in standing estrus (within 24-48 h after removal of controlled intravaginal drug releasing device) were inseminated (estrus = day 0) with frozen-thawed semen from a single ejaculate of a bull with proven fertility. Heifers from which an embryo was recovered (after slaughter on day 7) were classified as either having a viable embryo (morula/blastocyst stage) or a degenerate embryo (arrested at the 2- to 16-cell stage). The overall recovery rate (viable and degenerate combined) was 64%. Global liquid chromatography coupled to tandem mass spectrometry proteomic analysis of the histotroph collected identified 40 high-confidence proteins present on day 7; 26 proteins in the viable group, 10 in the degenerate group, and 4 shared between both groups. Five proteins (platelet-activating factor acetylhydrolase IB subunit γ [PAFAH1B3], tubulin α-1D chain, tubulin β-4A chain, cytochrome C, and dihydropyrimidinase-related protein-2) were unique or more abundant in the histotroph collected from animals with a viable embryo, and 1 protein (S100-A4) was more abundant in the histotroph collected from animals with a degenerate embryo. Of interest, PAFAH1B3, detected only in histotroph from the group yielding viable embryos, belongs to the group of platelet-activating factors that are known to be important for the development of the pre-implantation embryo in other species. To our knowledge this is the first report of PAFAH1B3 in relation to bovine early embryonic development.
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Full list of authors on original publication

M A Crowe, A. C. Evans, M. G. Diskin, M. Hilliard, G. Elia, M. P. Mullen, M E Beltman

Experts in our system

1
M A Crowe
Teagasc
Total Publications: 102
 
2
A.C.O. Evans
University College Dublin
Total Publications: 93
 
3
M G Diskin
Teagasc
Total Publications: 94
 
4
Giuliano Elia
Teagasc
Total Publications: 21
 
5
Michael P. Mullen
Teagasc
Total Publications: 32
 
6
Marijke Eileen Beltman
University College Dublin
Total Publications: 24