Type

Journal Article

Authors

Sinead M. Waters
M G Keane
Sean Doyle
Kay Ohlendieck
David A. Kenny
Sarah M Keady

Subjects

Biochemistry

Topics
1 protein mass spectrometry muscle skeletal real time genetic merit gene expression heat shock protein two dimensional difference gel electrophoresis citric acid cycle

Proteomic Profiling of Bovine M. longissimus lumborum from Crossbred Aberdeen Angus and Belgian Blue Sired Steers Varying in Genetic Merit for Carcass Weight. (2013)

Abstract Bovine skeletal muscle is a tissue of significant value to the beef industry and global economy. Proteomic analyses offer the opportunity to detect molecular mechanisms regulating muscle growth and intramuscular fat accumulation. The current study aimed to investigate differences in protein abundance in skeletal muscle tissue of cattle from two breeds of contrasting maturity (early v. late maturing), adiposity and muscle growth potential viz. Belgian Blue (BB) × Holstein Friesian and Aberdeen Angus (AA) × Holstein Friesian. Twenty AA (n=10) and BB (n=10) sired steers, the progeny of sires of either high (H) or low (L) genetic merit, expressed as expected progeny difference for carcass weight (EPD(cwt)), and bred through artificial insemination, were evaluated as four genetic groups viz. BBH, BBL, AAH, and AAL (n=5 per treatment). Chemical composition analysis of M. longissimus lumborum showed higher protein and moisture, and lower lipid concentrations for BB compared to AA sired steers. To investigate the effects of both sire breed and EPD(cwt) on M. longissimus lumborum, proteomic analysis was performed using two-dimensional (2-D) difference gel electrophoresis followed by mass spectrometry. Proteins were identified from their peptide sequences, using the NCBI and Swiss-prot databases. Metabolic enzymes involved in glycolysis (glycogen phosphorylase, phosphoglycerate mutase) and the citric acid cycle (aconitase-2, oxoglutarate dehydrogenase) were increased in AA v. BB sired steers. Expression of proteins involved in cell structure such as myosin light chain isoforms and troponins I and T were also altered due to sire breed. Furthermore, heat shock protein β1 and peroxiredoxin 6 involved in cell defence had increased abundance in muscle of AA relative BB sired steers. Protein abundance of glucose-6-phosphate isomerase, enolase-3 and pyruvate kinase was higher in AA sired animals of H compared to L EPD(cwt). Changes in the expression of these proteins were supported by gene expression analysis using qRT-PCR. This information will aid in our understanding of genetic influences controlling muscle growth and fat accumulation, and could contribute to future breeding programmes to increase lean tissue gain of beef cattle.
Collections Ireland -> Teagasc -> PubMed
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering: Biology
Ireland -> Maynooth University -> Type = Article
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering
Ireland -> Maynooth University -> Status = Published
Ireland -> Maynooth University -> Open Access DRIVERset

Full list of authors on original publication

Sinead M. Waters, M G Keane, Sean Doyle, Kay Ohlendieck, David A. Kenny, Sarah M Keady

Experts in our system

1
Sinead M. Waters
Teagasc
Total Publications: 99
 
2
Michael G. Keane
Teagasc
Total Publications: 50
 
3
Sean Doyle
Maynooth University
Total Publications: 130
 
4
Kay Ohlendieck
Maynooth University
Total Publications: 131
 
5
D.A. Kenny
Teagasc
Total Publications: 147