Type

Journal Article

Authors

R P Ross
P W O'Toole
C Stanton
C Hill
M Coakley
P M O'Connor
C M Guinane
B Lakshminarayanan

Subjects

Microbiology

Topics
lactic acid bacteria enterococcus faecium streptococcus mutans lactobacillus acidophilus listeria innocua irish intestinal microbiota lactobacillus salivarius

Isolation and characterization of bacteriocin-producing bacteria from the intestinal microbiota of elderly Irish subjects. (2012)

Abstract AIMS: To isolate and characterize bacteriocins produced by predominant species of lactic acid bacteria from faeces of elderly subjects. METHODS AND RESULTS: Screening over 70 000 colonies, from faecal samples collected from 266 subjects, using the indicator organisms Lactobacillus bulgaricus LMG 6901 and Listeria innocua DPC 3572, identified 55 antimicrobial-producing bacteria. Genomic fingerprinting following ApaI digestion revealed 15 distinct strains. The antimicrobial activities associated with 13 of the 15 strains were sensitive to protease treatment. The predominant antimicrobial-producing species were identified as Lactobacillus salivarius, Lactobacillus gasseri, Lactobacillus acidophilus, Lactobacillus crispatus and Enterococcus spp. A number of previously characterized bacteriocins, including ABP-118 and salivaricin B (from Lact. salivarius), enterocin B (Enterococcus faecium), lactacin B (Lact. acidophilus), gassericin T and a variant of gassericin A (Lact. gasseri), were identified. Interestingly, two antimicrobial-producing species, not generally associated with intestinally derived microorganisms were also isolated: Lactococcus lactis producing nisin Z and Streptococcus mutans producing mutacin II. CONCLUSION: These data suggest that bacteriocin production by intestinal isolates against our chosen targets under the screening conditions used was not frequent (0·08%). SIGNIFICANCE AND IMPACT OF THE STUDY: The results presented are important due to growing evidence indicating bacteriocin production as a potential probiotic trait by virtue of strain dominance and/or pathogen inhibition in the mammalian intestine.
Collections Ireland -> Teagasc -> PubMed

Full list of authors on original publication

R P Ross, P W O'Toole, C Stanton, C Hill, M Coakley, P M O'Connor, C M Guinane, B Lakshminarayanan

Experts in our system

1
R Paul Ross
Teagasc
Total Publications: 441
 
2
P W O'Toole
University College Cork
Total Publications: 72
 
3
Catherine Stanton
Teagasc
Total Publications: 261
 
4
Colin Hill
University College Cork
Total Publications: 351
 
5
Paula M. O'Connor
Teagasc
Total Publications: 85
 
6
Caitriona M Guinane
Teagasc
Total Publications: 35