Type

Journal Article

Authors

R P Ross
C Stanton
C Hill
G F Fitzgerald
P M O'Connor
C M Guinane
R M Kent

Subjects

Chemistry

Topics
antimicrobial peptides antimicrobial activity bioactive peptides production mass spectrometry maldi tof infant formula bacillus cereus

Production of the antimicrobial peptides Caseicin A and B by Bacillus isolates growing on sodium caseinate. (2012)

Abstract The aim of this study was to identify Bacillus isolates capable of degrading sodium caseinate and subsequently to generate bioactive peptides with antimicrobial activity. Sodium caseinate (2.5% w/v) was inoculated separately with 16 Bacillus isolates and allowed to ferment overnight. Protein breakdown in the fermentates was analysed using gel permeation-HPLC (GP-HPLC) and screened for peptides (<3-kDa) with MALDI-TOF mass spectrometry. Caseicin A (IKHQGLPQE) and caseicin B (VLNENLLR), two previously characterized antimicrobial peptides, were identified in the fermentates of both Bacillus cereus and Bacillus thuringiensis isolates. The caseicin peptides were subsequently purified by RP-HPLC and antimicrobial assays indicated that the peptides maintained the previously identified inhibitory activity against the infant formula pathogen Cronobacter sakazakii. We report a new method using Bacillus sp. to generate two previously characterized antimicrobial peptides from casein. This study highlights the potential to exploit Bacillus sp. or the enzymes they produce for the generation of bioactive antimicrobial peptides from bovine casein.
Collections Ireland -> Teagasc -> PubMed

Full list of authors on original publication

R P Ross, C Stanton, C Hill, G F Fitzgerald, P M O'Connor, C M Guinane, R M Kent

Experts in our system

1
R Paul Ross
Teagasc
Total Publications: 441
 
2
Catherine Stanton
Teagasc
Total Publications: 261
 
3
Colin Hill
University College Cork
Total Publications: 351
 
4
Gerald F Fitzgerald
Teagasc
Total Publications: 207
 
5
Paula M. O'Connor
Teagasc
Total Publications: 85
 
6
Caitriona M Guinane
Teagasc
Total Publications: 35