Type

Journal Article

Authors

Sinead M. Waters
Matthew S McCabe
Sukkyan Han
David A. Kenny
Ciara A Carberry

Subjects

Veterinary

Topics
animal nutritional physiological phenomena denaturing gradient gel electrophoresis polymerase chain reaction silage ciliophora fibrobacter prevotella rumen bacteria animals parasitology fermentation cattle genetics microbiology metabolism diet

Effect of phenotypic residual feed intake and dietary forage content on the rumen microbial community of beef cattle. (2012)

Abstract Feed-efficient animals have lower production costs and reduced environmental impact. Given that rumen microbial fermentation plays a pivotal role in host nutrition, the premise that rumen microbiota may contribute to host feed efficiency is gaining momentum. Since diet is a major factor in determining rumen community structure and fermentation patterns, we investigated the effect of divergence in phenotypic residual feed intake (RFI) on ruminal community structure of beef cattle across two contrasting diets. PCR-denaturing gradient gel electrophoresis (DGGE) and quantitative PCR (qPCR) were performed to profile the rumen bacterial population and to quantify the ruminal populations of Entodinium spp., protozoa, Fibrobacter succinogenes, Ruminococcus flavefaciens, Ruminococcus albus, Prevotella brevis, the genus Prevotella, and fungi in 14 low (efficient)- and 14 high (inefficient)-RFI animals offered a low-energy, high-forage diet, followed by a high-energy, low-forage diet. Canonical correspondence and Spearman correlation analyses were used to investigate associations between physiological variables and rumen microbial structure and specific microbial populations, respectively. The effect of RFI on bacterial profiles was influenced by diet, with the association between RFI group and PCR-DGGE profiles stronger for the higher forage diet. qPCR showed that Prevotella abundance was higher (P < 0.0001) in inefficient animals. A higher (P < 0.0001) abundance of Entodinium and Prevotella spp. and a lower (P < 0.0001) abundance of Fibrobacter succinogenes were observed when animals were offered the low-forage diet. Thus, differences in the ruminal microflora may contribute to host feed efficiency, although this effect may also be modulated by the diet offered.
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Full list of authors on original publication

Sinead M. Waters, Matthew S McCabe, Sukkyan Han, David A. Kenny, Ciara A Carberry

Experts in our system

1
Sinead M. Waters
Teagasc
Total Publications: 99
 
2
Matthew S. McCabe
Teagasc
Total Publications: 45
 
3
D.A. Kenny
Teagasc
Total Publications: 147