Type

Journal Article

Authors

R Paul Ross
Catherine Stanton
Gerald F Fitzgerald
Colin Hill
Sarah Norberg
Robert M. Kent
Caitriona M Guinane

Subjects

Biochemistry

Topics
phylogeny dna transposable elements genetics chromosome inversion classification physiology lactobacillus bacteriophages

Host specific diversity in Lactobacillus johnsonii as evidenced by a major chromosomal inversion and phage resistance mechanisms. (2010)

Abstract Genetic diversity and genomic rearrangements are a driving force in bacterial evolution and niche adaptation. We sequenced and annotated the genome of Lactobacillus johnsonii DPC6026, a strain isolated from the porcine intestinal tract. Although the genome of DPC6026 is similar in size (1.97 mbp) and GC content (34.8%) to the sequenced human isolate L. johnsonii NCC 533, a large symmetrical inversion of approximately 750 kb differentiated the two strains. Comparative analysis among 12 other strains of L. johnsonii including 8 porcine, 3 human and 1 poultry isolate indicated that the genome architecture found in DPC6026 is more common within the species than that of NCC 533. Furthermore a number of unique features were annotated in DPC6026, some of which are likely to have been acquired by horizontal gene transfer (HGT) and contribute to protection against phage infection. A putative type III restriction-modification system was identified, as were novel Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) elements. Interestingly, these particular elements are not widely distributed among L. johnsonii strains. Taken together these data suggest intra-species genomic rearrangements and significant genetic diversity within the L. johnsonii species and indicate towards a host-specific divergence of L. johnsonii strains with respect to genome inversion and phage exposure.
Collections Ireland -> Teagasc -> PubMed

Full list of authors on original publication

R Paul Ross, Catherine Stanton, Gerald F Fitzgerald, Colin Hill, Sarah Norberg, Robert M. Kent, Caitriona M Guinane

Experts in our system

1
R Paul Ross
Teagasc
Total Publications: 441
 
2
Catherine Stanton
Teagasc
Total Publications: 261
 
3
Gerald F Fitzgerald
Teagasc
Total Publications: 207
 
4
Colin Hill
University College Cork
Total Publications: 351
 
5
Robert M. Kent
University College Cork
Total Publications: 7
 
6
Caitriona M Guinane
Teagasc
Total Publications: 35