Type

Journal Article

Authors

R Paul Ross
Colin Hill
Barry Kiely
Fergus Shanahan
Paul D. Cotter
Fiona Fouhy
Fiona Crispie
Orla O'Sullivan
Alleson Dobson
Mary C. Rea

Subjects

Microbiology

Topics
drug effects feces metagenome bacteriocins models biological thuricin molecular sequence data colon metronidazole humans vancomycin pharmacology anti bacterial agents sequence analysis dna clostridium difficile base sequence genetics lacticin 481 microbiology

Effect of broad- and narrow-spectrum antimicrobials on Clostridium difficile and microbial diversity in a model of the distal colon. (2010)

Abstract Vancomycin, metronidazole, and the bacteriocin lacticin 3147 are active against a wide range of bacterial species, including Clostridium difficile. We demonstrate that, in a human distal colon model, the addition of each of the three antimicrobials resulted in a significant decrease in numbers of C. difficile. However, their therapeutic use in the gastrointestinal tract may be compromised by their broad spectrum of activity, which would be expected to significantly impact on other members of the human gut microbiota. We used high-throughput pyrosequencing to compare the effect of each antimicrobial on the composition of the microbiota. All three treatments resulted in a decrease in the proportion of sequences assigned to the phyla Firmicutes and Bacteroidetes, with a corresponding increase in those assigned to members of the Proteobacteria. One possible means of avoiding such "collateral damage" would involve the application of a narrow-spectrum antimicrobial with specific anti-C. difficile activity. We tested this hypothesis using thuricin CD, a narrow-spectrum bacteriocin produced by Bacillus thuringiensis, which is active against C. difficile. The results demonstrated that this bacteriocin was equally effective at killing C. difficile in the distal colon model but had no significant impact on the composition of the microbiota. This offers the possibility of developing a targeted approach to eliminating C. difficile in the colon, without collateral damage.
Collections Ireland -> Teagasc -> PubMed

Full list of authors on original publication

R Paul Ross, Colin Hill, Barry Kiely, Fergus Shanahan, Paul D. Cotter, Fiona Fouhy, Fiona Crispie, Orla O'Sullivan, Alleson Dobson, Mary C. Rea

Experts in our system

1
R Paul Ross
Teagasc
Total Publications: 441
 
2
Colin Hill
University College Cork
Total Publications: 351
 
3
Barry Kiely
University College Cork
Total Publications: 21
 
4
Fergus Shanahan
University College Cork
Total Publications: 237
 
5
Paul D. Cotter
Teagasc
Total Publications: 253
 
6
Fiona Fouhy
Teagasc
Total Publications: 27
 
7
Fiona Crispie
Teagasc
Total Publications: 30
 
8
Orla O'Sullivan
Teagasc
Total Publications: 92
 
9
Mary C. Rea
Teagasc
Total Publications: 68