Type

Journal Article

Authors

Colin Hill
R Paul Ross
John C Vederas
Jing Zheng
Randy M Whittal
Paula M. O'Connor
Evelyn Clayton
Clarissa S Sit
Mary C. Rea

Subjects

Microbiology

Topics
humans protein processing post translational pharmacology tandem mass spectrometry anti bacterial agents s adenosylmethionine chemistry clostridium difficile amino acid sequence sequence analysis protein genetics microbiology drug effects feces bacteriocins thuricin molecular sequence data bacillus thuringiensis nuclear magnetic resonance biomolecular

Thuricin CD, a posttranslationally modified bacteriocin with a narrow spectrum of activity against Clostridium difficile. (2010)

Abstract The last decade has seen numerous outbreaks of Clostridium difficile-associated disease (CDAD), which presented significant challenges for healthcare facilities worldwide. We have identified and purified thuricin CD, a two-component antimicrobial that shows activity against C. difficile in the nanomolar range. Thuricin CD is produced by Bacillus thuringiensis DPC 6431, a bacterial strain isolated from a human fecal sample, and it consists of two distinct peptides, Trn-alpha and Trn-beta, that act synergistically to kill a wide range of clinical C. difficile isolates, including ribotypes commonly associated with CDAD (e.g., ribotype 027). However, this bacteriocin thuricin CD has little impact on most other genera, including many gastrointestinal commensals. Complete amino acid sequencing using infusion tandem mass spectrometry indicated that each peptide is posttranslationally modified at its respective 21st, 25th, and 28th residues. Solution NMR studies on [(13)C,(15)N] Trn-alpha and [(13)C,(15)N]Trn-beta were used to characterize these modifications. Analysis of multidimensional NOESY data shows that specific cysteines are linked to the alpha-carbons of the modified residues, forming three sulfur to alpha-carbon bridges. Complete sequencing of the thuricin CD gene cluster revealed genes capable of encoding two S'-adenosylmethionine proteins that are characteristically associated with unusual posttranslational modifications. Thuricin CD is a two-component antimicrobial peptide system with sulfur to alpha-carbon linkages, and it may have potential as a targeted therapy in the treatment of CDAD while also reducing collateral impact on the commensal flora.
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Full list of authors on original publication

Colin Hill, R Paul Ross, John C Vederas, Jing Zheng, Randy M Whittal, Paula M. O'Connor, Evelyn Clayton, Clarissa S Sit, Mary C. Rea

Experts in our system

1
Colin Hill
University College Cork
Total Publications: 351
 
2
R Paul Ross
Teagasc
Total Publications: 441
 
3
Paula M. O'Connor
Teagasc
Total Publications: 85
 
4
Evelyn M Clayton
Teagasc
Total Publications: 7
 
5
Mary C. Rea
Teagasc
Total Publications: 68