Journal Article


D C Wathes
A Brass
D.A. Kenny
M. G. Diskin
J.J. Murphy
R Fitzpatrick
B Earley
J Patton
S D McCarthy
S M Waters
and 1 others



animals female cattle genetics immunology spleen postpartum period gene expression profiling metabolism blood cell count polymerase chain reaction energy metabolism oligonucleotide array sequence analysis immunity innate dairying gene expression regulation gene regulatory networks

Pleiotropic effects of negative energy balance in the postpartum dairy cow on splenic gene expression: repercussions for innate and adaptive immunity. (2009)

Abstract Increased energy demands to support lactation, coupled with lowered feed intake capacity results in negative energy balance (NEB) and is typically characterized by extensive mobilization of body energy reserves in the early postpartum dairy cow. The catabolism of stored lipid leads to an increase in the systemic concentrations of nonesterified fatty acids (NEFA) and beta-hydroxy butyrate (BHB). Oxidation of NEFA in the liver result in the increased production of reactive oxygen species and the onset of oxidative stress and can lead to disruption of normal metabolism and physiology. The immune system is depressed in the peripartum period and early lactation and dairy cows are therefore more vulnerable to bacterial infections causing mastitis and or endometritis at this time. A bovine Affymetrix oligonucleotide array was used to determine global gene expression in the spleen of dairy cows in the early postpartum period. Spleen tissue was removed post mortem from five severe NEB (SNEB) and five medium NEB (MNEB) cows 15 days postpartum. SNEB increased systemic concentrations of NEFA and BHB, and white blood cell and lymphocyte numbers were decreased in SNEB animals. A total of 545 genes were altered by SNEB. Network analysis using Ingenuity Pathway Analysis revealed that SNEB was associated with NRF2-mediated oxidative stress, mitochondrial dysfunction, endoplasmic reticulum stress, natural killer cell signaling, p53 signaling, downregulation of IL-15, BCL-2, and IFN-gamma; upregulation of BAX and CHOP and increased apoptosis with a potential negative impact on innate and adaptive immunity.
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Full list of authors on original publication

D C Wathes, A Brass, D.A. Kenny, M. G. Diskin, J.J. Murphy, R Fitzpatrick, B Earley, J Patton, S D McCarthy, S M Waters and 1 others

Experts in our system

D.A. Kenny
Total Publications: 147
M G Diskin
Total Publications: 94
J.J. Murphy
Total Publications: 45
Bernadette Earley
Total Publications: 120
Sinead M. Waters
Total Publications: 99