Type

Journal Article

Authors

Fergus Shanahan
Cormac G M Gahan
Reynolds P Ross
Colin Hill
Michael Cronin
Thomas L McDonald
Annika Weber
Pat G. Casey
Sarah O'Flaherty
Gillian E. Gardiner

Subjects

Microbiology

Topics
prevention control microbiology intestine small serum amyloid a protein immunology animals chemical synthesis colon enterobacteriaceae infections liver cell line salmonella typhimurium mice citrobacter rodentium enteritis isolation purification humans saa3p protein human peptides escherichia coli drug effects feces bacterial adhesion cecum

Evaluation of colostrum-derived human mammary-associated serum amyloid A3 (M-SAA3) protein and peptide derivatives for the prevention of enteric infection: in vitro and in murine models of intestinal disease. (2009)

Abstract In vitro experiments confirmed that a 10-mer peptide derived from human mammary-associated serum amyloid A3 (M-SAA3) protected intestinal epithelial cells from enteropathogenic Escherichia coli (EPEC) adherence. The entire 42-mer human M-SAA3 protein was even more effective, reducing EPEC binding by 72% relative to untreated cells (P<0.05), compared with 25% and 57% reductions for the human 10-mer and Lactobacillus GG, respectively. However, none of the M-SAA3 peptides reduced Salmonella invasion in vitro (P>0.05). Each of the M-SAA3 10-mer peptides and the 42-mer was then administered orally to mice at 500 mug day(-1) for 4 days before deliberate infection with either Citrobacter rodentium (mouse model of EPEC) or Salmonella Typhimurium. None of the peptides protected against Salmonella infection and the 42-mer may even increase infection, as there was a trend towards increased Salmonella counts in the liver and small intestine in 42-mer-treated mice compared with those in sodium acetate-treated control mice. Citrobacter counts were reduced in the caecum of mice administered the 42-mer relative to a scrambled 10-mer (P<0.05), but not compared with the sodium acetate control and no reductions were observed in the faeces or colon. Overall, although promising anti-infective activity was demonstrated in vitro, neither the 42-mer M-SAA3 protein nor a 10-mer peptide derivative prevented enteric infection in the animal models tested.
Collections Ireland -> Teagasc -> PubMed

Full list of authors on original publication

Fergus Shanahan, Cormac G M Gahan, Reynolds P Ross, Colin Hill, Michael Cronin, Thomas L McDonald, Annika Weber, Pat G. Casey, Sarah O'Flaherty, Gillian E. Gardiner

Experts in our system

1
Fergus Shanahan
University College Cork
Total Publications: 237
 
2
Cormac G M Gahan
University College Cork
Total Publications: 109
 
3
R Paul Ross
Teagasc
Total Publications: 441
 
4
Colin Hill
University College Cork
Total Publications: 351
 
5
Pat G. Casey
University College Cork
Total Publications: 40
 
6
Gillian E. Gardiner
Teagasc
Total Publications: 55