Type

Journal Article

Authors

D.A. Kenny
A. A. Hennessy
P. Lonergan
J M Sreenan
C O Lynch
F Carter
S Childs

Subjects

Biochemistry

Topics
drug effects fatty acids fatty acids omega 3 embryo mammalian blood embryology quality control physiology metabolism pharmacology dietary supplements animals therapeutic irrigation estrus synchronization uterus female cytology cattle chemistry superovulation gene expression regulation developmental

Embryo yield and quality following dietary supplementation of beef heifers with n-3 polyunsaturated fatty acids (PUFA). (2008)

Abstract The objective of this study was to examine the effects of dietary n-3 polyunsaturated fatty acid (n-3 PUFA) supplementation on embryo yield and quality in heifers. Animals were individually offered barley straw and concentrate diets supplemented with either palmitic acid (C16:0; CON) or a partially rumen protected n-3 PUFA-enriched supplement. Following oestrous cycle synchronisation, superovulation was induced using FSH. Blood samples were collected for the measurement of fatty acids, metabolites, insulin and IGF-1. On day 7 post-insemination the number of ovulations was estimated and embryos recovered non-surgically and quality graded. At embryo recovery 50 ml of the uterine flushing was collected from each horn for fatty acid analysis. Grade 1 embryos were isolated, snap frozen in liquid nitrogen and stored at -80 degrees C. mRNA expression for six genes, LIF, BAX, Cx43 and E-CAD associated with embryo development, and PPAR-alpha and -delta, associated with lipid metabolism was analysed. The n-3 PUFA supplementation increased plasma n-3 PUFA concentration (P<0.05) and reduced n-6:n-3 PUFA ratio (P<0.05). Uterine concentration of the n-3 PUFA, eicosapentaenoic acid was increased (P<0.05) and the concentration of arachidonic acid decreased (P<0.05) following n-3 PUFA supplementation. While CON increased triglyceride concentrations, diet did not affect the other plasma metabolites, insulin or IGF-1 (P>0.05). Similarly, there was no effect of diet on superovulation rate, embryo recovery rate, embryo quality (P>0.05) or mRNA expression of the genes examined (P>0.05).
Collections Ireland -> Teagasc -> PubMed

Full list of authors on original publication

D.A. Kenny, A. A. Hennessy, P. Lonergan, J M Sreenan, C O Lynch, F Carter, S Childs

Experts in our system

1
D.A. Kenny
Teagasc
Total Publications: 138
 
2
A. A. Hennessy
Teagasc
Total Publications: 19
 
3
P. Lonergan
University College Dublin
Total Publications: 190
 
4
F. Carter
University College Dublin
Total Publications: 19