Type

Journal Article

Authors

R Paul Ross
Olivia McAuliffe
Gerald F Fitzgerald
Colin Hill
Aidan Coffey
Susan Mills

Subjects

Biochemistry

Topics
cloning molecular cation transport proteins cobalt genetics metabolism classification plasmids magnesium biological transport active growth development open reading frames pharmacology bacterial proteins recombinant proteins cora protein salmonella gene expression regulation bacterial mutagenesis insertional lactococcus

Insertional inactivation of determinants for Mg2+ and Co2+ transport as a tool for screening recombinant Lactococcus species clones. (2005)

Abstract Insertional inactivation of the plasmid-encoded determinants for Mg(2+) and Co(2+) transport, orf18/corA, provides a tool for screening recombinant clones in Lactococcus, based on the observation that overexpression of orf18/corA results in cell growth inhibition on certain concentrations of CoCl(2). The lacticin 3147 immunity gene, ltnI, was used to insertionally inactivate orf18/corA. The resulting clones were capable of growth on concentrations of CoCl(2) that were inhibitory to the parent strain. Since only 3 of 17 lactococcal starters naturally harbor corA, the system has potential as a screen for selecting recombinant lactococcal clones.
Collections Ireland -> Teagasc -> PubMed

Full list of authors on original publication

R Paul Ross, Olivia McAuliffe, Gerald F Fitzgerald, Colin Hill, Aidan Coffey, Susan Mills

Experts in our system

1
R Paul Ross
Teagasc
Total Publications: 441
 
2
Olivia McAuliffe
Teagasc
Total Publications: 64
 
3
Gerald F Fitzgerald
Teagasc
Total Publications: 207
 
4
Colin Hill
University College Cork
Total Publications: 351
 
5
Aidan Coffey
Teagasc
Total Publications: 63
 
6
Susan Mills
Teagasc
Total Publications: 15