Type

Journal Article

Authors

R Paul Ross
Catherine Stanton
Gerald F Fitzgerald
Colin Hill
Peadar G Lawlor
P Brendan Lynch
Garrett Casey
Pat G. Casey
Gillian E. Gardiner

Subjects

Microbiology

Topics
growth development male random amplified polymorphic dna technique dna bacterial lactobacillus dna fingerprinting pathogenicity pediococcus enterobacteriaceae digestive system gastric juice female microbiology sus scrofa genetics administration dosage isolation purification animals colony count microbial probiotics administration oral

Relative ability of orally administered Lactobacillus murinus to predominate and persist in the porcine gastrointestinal tract. (2004)

Abstract Five porcine-derived Lactobacillus or Pediococcus isolates administered to pigs (n = 4), either singly or as a combination at approximately 10(10) CFU per day varied with respect to intestinal survival and persistence. Two Lactobacillus murinus strains survived best and were excreted at approximately 10(7) to 10(8) CFU/g of feces. In contrast, Pediococcus pentosaceus DPC6006 had the lowest fecal count at approximately 10(5) CFU/g and was excreted at a significantly lower level than both L. murinus strains. Fecal L. murinus DPC6003 counts were also significantly higher than both Lactobacillus salivarius DPC6005 and Lactobacillus pentosus DPC6004 ( approximately 10(6) CFU/g). The L. murinus strains persisted for at least 9 days postadministration in both the feces and the cecum. Animals fed a combination of all five strains excreted approximately 10(7) CFU of the administered strains/g, with L. murinus predominating, as determined by randomly amplified polymorphic DNA PCR. Postadministration, variation was observed between animals fed the strain combination, but in general, L. murinus DPC6002 and DPC6003 and L. pentosus DPC6004 predominated in the feces and the cecum while P. pentosaceus DPC6006 was detected only in the cecum. Fifteen days after the start of culture administration, mean fecal Enterobacteriaceae counts were significantly lower in some of the treatment groups. In addition, when mean preadministration counts were compared with those obtained after 21 days of culture administration, Enterobacteriaceae counts were reduced by approximately 87 to 98% in pigs fed L. salivarius DPC6005, P. pentosaceus DPC6006, L. pentosus DPC6004, and the culture mix. In conclusion, the porcine intestinal isolates have potential as probiotic feed additives for pigs, with differences in strain performance highlighting the advantages of using culture combinations.
Collections Ireland -> Teagasc -> PubMed

Full list of authors on original publication

R Paul Ross, Catherine Stanton, Gerald F Fitzgerald, Colin Hill, Peadar G Lawlor, P Brendan Lynch, Garrett Casey, Pat G. Casey, Gillian E. Gardiner

Experts in our system

1
R Paul Ross
Teagasc
Total Publications: 441
 
2
Catherine Stanton
Teagasc
Total Publications: 261
 
3
Gerald F Fitzgerald
Teagasc
Total Publications: 207
 
4
Colin Hill
University College Cork
Total Publications: 351
 
6
Pat G. Casey
University College Cork
Total Publications: 40
 
7
Gillian E. Gardiner
Teagasc
Total Publications: 55