Type

Journal Article

Authors

C Hill
R P Ross
D P Twomey
R M Hickey

Subjects

Microbiology

Topics
bacteriocins biosynthesis dna bacterial gene expression regulation bacterial physiology enterocin a bacterial proteins genes regulator escherichia coli genes bacterial genetic vectors anti bacterial agents immunology transformation genetic lacticin 481 enterococcus faecium genetics genes reporter plasmids enzymology glucuronidase

Potential of the enterocin regulatory system to control expression of heterologous genes in Enterococcus. (2003)

Abstract To exploit the enterocin regulatory system for regulated expression of genes in Enterococcus. Production of some pediocin-like bacteriocins such as enterocin A in Enterococcus is regulated by a three-component system comprising a histidine kinase (entK ), a response regulator (entR) and an induction factor (entF ). Exposure to the induction factor results in the transcription of gene(s) under the control of the enterocin A promoter, including entA which encodes the bacteriocin. In an effort to exploit this system for expression of genes in Enterococcus, a number of vectors were constructed which contain the entA promoter followed by convenient cloning sites to introduce gene(s) of interest. These vectors were used in an enterococcal background which does not produce induction factor but does produce both the kinase and regulator proteins. The system was tested using the reporter genes ltnI (lacticin 3147 immunity) and gusA (beta-glucuronidase) under the control of the entA promoter. Upon addition of the induction factor, the beta-glucuronidase activity increased 20-fold when compared with uninduced cells. In addition, concentrations of as little as 0.2 nm synthetic EntF were sufficient to give maximal expression. The potential benefit of having an expression system based on EntF is that gene expression can be finely controlled upon addition of low concentrations of a peptide that can easily be artificially synthesized.
Collections Ireland -> Teagasc -> PubMed

Full list of authors on original publication

C Hill, R P Ross, D P Twomey, R M Hickey

Experts in our system

1
Colin Hill
University College Cork
Total Publications: 351
 
2
R Paul Ross
Teagasc
Total Publications: 441
 
3
Rita M. Hickey
Teagasc
Total Publications: 24