Journal Article


N Leonard
G Kelly
B Earley
J J Sheridan
D J Bolton
P McGee



colony count microbial fresh water agriculture escherichia coli o157 pharmacology feces drug effects bacteria transmission microbiology cattle animals metabolism anti bacterial agents microbial sensitivity tests water supply escherichia coli infections isolation purification growth development

Survival of Escherichia coli O157:H7 in farm water: its role as a vector in the transmission of the organism within herds. (2002)

Abstract The study aimed to investigate the survival characteristics of Escherichia coli O157:H7 in farm water (FW), and in sterile distilled municipal water (SDW), stored outdoors under field conditions, with or without the addition of faeces (1% w/v), in a farmyard shed and the laboratory at 15 degrees C. Water samples were inoculated with E. coli O157:H7 at 10(3) and 10(6) ml(-1), and sampled over a 31-day period. In FW stored outdoors in a field, E. coli O157:H7 survived for 14 days at temperatures <15 degrees C, at both inoculation levels, while in the laboratory at 15 degrees C, the organism was still detectable at low levels (<1 log10 cfu ml(-1)) after 31 days. The addition of bovine faeces to water outdoors (1% w/v) resulted in survival for 24 days. In SDW inoculated at 10(6) ml(-1) and stored in the laboratory (15 degrees C), only a 2.5 log reduction was observed after 31 days, while the organism could not be detected after 17 days in the field. Preliminary screening of water samples stored outdoors isolated a bacterium which exhibited antimicrobial activity towards E. coli O157:H7. The survival of E. coli O157:H7 observed in this study illustrates the potential of farm water to act as a vehicle in the transfer of the organism across a herd. The difficulty in extrapolating results from controlled laboratory situations to on-farm conditions is also highlighted in this study.
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Full list of authors on original publication

N Leonard, G Kelly, B Earley, J J Sheridan, D J Bolton, P McGee

Experts in our system

Bernadette Earley
Total Publications: 120
D J Bolton