Journal Article


C Hill
R P Ross
O McAuliffe
M P Ryan



enterococcus faecalis sequence alignment amino acid sequence horses genes bacterial molecular sequence data biosynthesis cytolysin enterococcus faecalis microbial sensitivity tests protein processing post translational pharmacology bacteria transformation bacterial animals bacteriocins lacticin 481 drug effects electroporation hemolysis lactococcus lactis bacterial proteins gene duplication metabolism recombinant proteins cytotoxins genetics chemistry

Heterologous expression of lacticin 3147 in Enterococcus faecalis: comparison of biological activity with cytolysin. (2001)

Abstract Lacticin 3147 is a broad-spectrum, two-component, lanthionine-containing bacteriocin produced by Lactococcus lactis DPC3147 which has widespread food and biomedical applications as a natural antimicrobial. Other two-component lantibiotics described to date include cytolysin and staphylococcin C55. Interestingly, cytolysin, produced by Enterococcus faecalis, has an associated haemolytic activity. The objective of this study was to compare the biological activity of lacticin 3147 with cytolysin. The lacticin 3147-encoding determinants were heterologously expressed in Ent. faecalis FA2-2, a plasmid-free strain, to generate Ent. faecalis pOM02, thereby facilitating a direct comparison with Ent. faecalis FA2-2.pAD1, a cytolysin producer. Both heterologously expressed lacticin 3147 and cytolysin exhibited a broad spectrum of activity against bacterial targets. Furthermore, enterococci expressing active lacticin 3147 did not exhibit a haemolytic activity against equine blood cells. The results thus indicate that the lacticin 3147 biosynthetic machinery can be heterologously expressed in an enterococcal background resulting in the production of the bacteriocin with no detectable haemolytic activity.
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Full list of authors on original publication

C Hill, R P Ross, O McAuliffe, M P Ryan

Experts in our system

Colin Hill
University College Cork
Total Publications: 351
R Paul Ross
Total Publications: 441
Olivia McAuliffe
Total Publications: 64
Michael P. Ryan
University College Dublin
Total Publications: 80