Type

Journal Article

Authors

Lidia Tajber
Owen I. Corrigan
Carlos Medina
Maria Jose Santos-Martinez
Krzysztof Jan Paluch
Anita Umerska
Carlos Medina Martin

Subjects

Chemistry

Topics
nanoscience materials salmon calcitonin zeta potential delivery x ray diffraction extended self investigations self assembly caco 2 cells self assembled peptide

Chondroitin-based nanoplexes as peptide delivery systems - Investigations into the self-assembly process, solid-state and extended release characteristics. (2015)

Abstract A new type of self-assembled polyelectrolyte complex nanocarrier composed of chondroitin (CHON) and protamine (PROT) was designed and the ability of the carriers to bind salmon calcitonin (sCT) was examined. The response of sCT-loaded CHON/PROT NPs to a change in the properties of the liquid medium, e.g. its pH, composition or ionic strength was studied and in vitro peptide release was assessed. The biocompatibility of the NPs was evaluated in Caco-2 cells. CHON/PROT NPs were successfully obtained with properties that were dependent on the concentration of the polyelectrolytes and their mixing ratio. X-ray diffraction determined the amorphous nature of the negatively charged NPs, while those with the positive surface potential were semi-crystalline. sCT was efficiently associated with the nanocarriers (98-100%) and a notably high drug loading (13-38%) was achieved. The particles had negative zeta potential values and were homogenously dispersed with sizes between 60 and 250nm. CHON/PROT NPs released less than 10% of the total loaded peptide in the first hour of the in vitro release studies. The enthalpy of the decomposition exotherm correlated with the amount of sCT remaining in NPs after the release experiments. The composition of medium and its ionic strength was found to have a considerable influence on the release of sCT from CHON/PROT NPs. Complexation to CHON markedly reduced the toxic effects exerted by PROT and the NPs were compatible and well tolerated by Caco-2 cells.
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Full list of authors on original publication

Lidia Tajber, Owen I. Corrigan, Carlos Medina, Maria Jose Santos-Martinez, Krzysztof Jan Paluch, Anita Umerska, Carlos Medina Martin

Experts in our system

1
Lidia Tajber
Trinity College Dublin
Total Publications: 83
 
2
Owen I. Corrigan
Trinity College Dublin
Total Publications: 66
 
3
Carlos Medina
Trinity College Dublin
Total Publications: 23
 
4
Maria Jose Santos-Martinez
Trinity College Dublin
Total Publications: 29
 
5
Krzysztof J. Paluch
Trinity College Dublin
Total Publications: 28
 
6
Anita Umerska
Trinity College Dublin
Total Publications: 8
 
7
Carlos Medina Martin
Trinity College Dublin
Total Publications: 28