Journal Article


Joan A Geoghegan
Kieran Wynne
Dara P O'Halloran



signal staphylococcus aureus mrsa culture molecular biology immune evasion microbiology molecular genetics genetics immunology inflammation infection protein binding protein genes society protein sdre blood staphylococcus aureus

Protein A Is Released into the Staphylococcus aureus Culture Supernatant with an Unprocessed Sorting Signal. (2015)

Abstract The immunoglobulin binding protein A (SpA) of Staphylococcus aureus is synthesized as a precursor with a C-terminal sorting signal. The sortase A enzyme mediates covalent attachment to peptidoglycan so that SpA is displayed on the surface of the bacterium. Protein A is also found in the extracellular medium, but the processes involved in its release are not fully understood. Here, we show that a portion of SpA is released into the supernatant with an intact sorting signal, indicating that it has not been processed by sortase A. Release of SpA was reduced when the native sorting signal of SpA was replaced with the corresponding region of another sortase-anchored protein (SdrE). Similarly, a reporter protein fused to the sorting signal of SpA was released to a greater extent than the same polypeptide fused to the SdrE sorting signal. Released SpA protected bacteria from killing in human blood, indicating that it contributes to immune evasion.
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Full list of authors on original publication

Joan A Geoghegan, Kieran Wynne, Dara P O'Halloran

Experts in our system

Joan A Geoghegan
Trinity College Dublin
Total Publications: 44
Kieran Wynne
Royal College of Surgeons in Ireland
Total Publications: 45