Type

Journal Article

Authors

Marek W Radomski
Carlos Medina
Maria Jose Santos-Martinez
Iwona Inkielewicz-Stepniak
Carlos Medina Martin

Subjects

Pharmacology

Topics
nanoscience materials real time polymerase chain reaction silver exposure toxicological humans fibroblasts nanomedicine silver nanoparticles oxidative stress real time pcr sodium fluoride nanopharmacology gingiva drug effects metal nanoparticles apoptosis physiopathology human toxicity pathology drug combinations cell line mitogen activated protein kinases

Pharmacological and toxicological effects of co-exposure of human gingival fibroblasts to silver nanoparticles and sodium fluoride. (2014)

Abstract Silver nanoparticles (AgNPs) and fluoride (F) are pharmacological agents widely used in oral medicine and dental practice due to their anti-microbial/anti-cavity properties. However, risks associated with the co-exposure of local cells and tissues to these xenobiotics are not clear. Therefore, we have evaluated the effects of AgNPs and F co-exposure on human gingival fibroblast cells. Human gingival fibroblast cells (CRL-2014) were exposed to AgNPs and/or F at different concentrations for up to 24 hours. Cellular uptake of AgNPs was examined by transmission electron microscopy. Downstream inflammatory effects and oxidative stress were measured by real-time quantitative polymerase chain reaction (PCR) and reactive oxygen species (ROS) generation. Cytotoxicity and apoptosis were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and real-time quantitative PCR and flow cytometry, respectively. Finally, the involvement of mitogen-activated protein kinases (MAPK) was studied using Western blot. We found that AgNPs penetrated the cell membrane and localized inside the mitochondria. Co-incubation experiments resulted in increased oxidative stress, inflammation, and apoptosis. In addition, we found that co-exposure to both xenobiotics phosphorylated MAPK, particularly p42/44 MAPK. A combined exposure of human fibroblasts to AgNPs and F results in increased cellular damage. Further studies are needed in order to evaluate pharmacological and potentially toxicological effects of AgNPs and F on oral health.
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Full list of authors on original publication

Marek W Radomski, Carlos Medina, Maria Jose Santos-Martinez, Iwona Inkielewicz-Stepniak, Carlos Medina Martin

Experts in our system

1
Marek W Radomski
Trinity College Dublin
Total Publications: 44
 
2
Carlos Medina
Trinity College Dublin
Total Publications: 23
 
3
Maria Jose Santos-Martinez
Trinity College Dublin
Total Publications: 29
 
4
Carlos Medina Martin
Trinity College Dublin
Total Publications: 28