Type

Journal Article

Authors

Lorraine O'Driscoll
John Crown
Irene M Ghobrial
Martina Gogarty
Susan Breslin
Sweta Rani
Claire Corcoran

Subjects

Microbiology

Topics
micrornas administration dosage female quinazolines receptor igf type 1 erbb2 protein human mirn630 microrna human genetics lapatinib gene expression regulation neoplastic drug resistance neoplasm biosynthesis pathology cell proliferation drug effects breast neoplasms receptor erbb 2 quinolines anoikis humans

miR-630 targets IGF1R to regulate response to HER-targeting drugs and overall cancer cell progression in HER2 over-expressing breast cancer. (2013)

Abstract While the treatment of HER2 over-expressing breast cancer with recent HER-targeted drugs has been highly effective for some patients, primary (also known as innate) or acquired resistance limits the success of these drugs. microRNAs have potential as diagnostic, prognostic and predictive biomarkers, as well as replacement therapies. Here we investigated the role of microRNA-630 (miR-630) in breast cancer progression and as a predictive biomarker for response to HER-targeting drugs, ultimately yielding potential as a therapeutic approach to add value to these drugs. We investigated the levels of intra- and extracellular miR-630 in cells and conditioned media from breast cancer cell lines with either innate- or acquired- resistance to HER-targeting lapatinib and neratinib, compared to their corresponding drug sensitive cell lines, using qPCR. To support the role of miR-630 in breast cancer, we examined the clinical relevance of this miRNA in breast cancer tumours versus matched peritumours. Transfection of miR-630 mimics and inhibitors was used to manipulate the expression of miR-630 to assess effects on response to HER-targeting drugs (lapatinib, neratinib and afatinib). Other phenotypic changes associated with cellular aggressiveness were evaluated by motility, invasion and anoikis assays. TargetScan prediction software, qPCR, immunoblotting and ELISAs, were used to assess miR-630's regulation of mRNA, proteins and their phosphorylated forms. We established that introducing miR-630 into cells with innate- or acquired- resistance to HER-drugs significantly restored the efficacy of lapatinib, neratinib and afatinib; through a mechanism which we have determined to, at least partly, involve miR-630's regulation of IGF1R. Conversely, we demonstrated that blocking miR-630 induced resistance/insensitivity to these drugs. Cellular motility, invasion, and anoikis were also observed as significantly altered by miR-630 manipulation, whereby introducing miR-630 into cells reduced cellular aggression while inhibition of miR-630 induced a more aggressive cellular phenotype. Taken together, our findings suggest miR-630 as a key regulator of cancer cell progression in HER2 over-expressing breast cancer, through targeting of IGF1R. This study supports miR-630 as a diagnostic and a predictive biomarker for response to HER-targeted drugs and indicates that the therapeutic addition of miR-630 may enhance and improve patients' response to HER-targeting drugs.
Collections Ireland -> Trinity College Dublin -> PubMed

Full list of authors on original publication

Lorraine O'Driscoll, John Crown, Irene M Ghobrial, Martina Gogarty, Susan Breslin, Sweta Rani, Claire Corcoran

Experts in our system

1
Lorraine O'Driscoll
Trinity College Dublin
Total Publications: 164
 
2
John Crown
Dublin City University
Total Publications: 104
 
3
Susan Breslin
Trinity College Dublin
Total Publications: 8
 
4
Sweta Rani
Trinity College Dublin
 
5
Claire Corcoran
Trinity College Dublin