Type

Journal Article

Authors

Lorraine O'Driscoll
Ken O'Byrne
John Crown
Kathy Gately
Sweta Rani

Subjects

Medicine & Nursing

Topics
cancer biomarkers early diagnosis micrornas non small cell lung cancer nsclc global lung low density minimally invasive serum rna isolation adenocarcinoma

Global analysis of serum microRNAs as potential biomarkers for lung adenocarcinoma. (2013)

Abstract Early diagnosis and the ability to predict the most relevant treatment option for individuals is essential to improve clinical outcomes for non-small cell lung cancer (NSCLC) patients. Adenocarcinoma (ADC), a subtype of NSCLC, is the single biggest cancer killer and therefore an urgent need to identify minimally invasive biomarkers to enable early diagnosis. Recent studies, by ourselves and others, indicate that circulating miRNAs have potential as biomarkers. Here we applied global profiling approaches in serum from patients with ADC of the lung to explore if miRNAs have potential as diagnostic biomarkers. This study involved RNA isolation from 80 sera specimens including those from ADC patients (equal numbers of Stages 1, 2, 3, and 4) and age- and gender-matched controls (n = 40 each). 667 miRNAs were co-analyzed in these specimens using TaqMan low density arrays and qPCR validation using individual miRNAs. Overall, approximately 390 and 370 miRNAs were detected in ADC and control sera, respectively. A group of 6 miRNAs, miR-30c-1* (AUC = 0.74; p<0.002), miR-616* (AUC = 0.71; p = 0.001), miR-146b-3p (AUC = 0.82; p<0.0001), miR-566 (AUC = 0.80; p<0.0001), miR-550 (AUC = 0.72; p = 0.0006), and miR-939 (AUC = 0.82; p<0.0001) was found to be present at substantially higher levels in ADC compared with control sera. Conversely, miR-339-5p and miR-656 were detected at substantially lower levels in ADC sera (co-analysis resulting in AUC = 0.6; p = 0.02). Differences in miRNA profile identified support circulating miRNAs having potential as diagnostic biomarkers for ADC. More extensive studies of ADC and control serum specimens are warranted to independently validate the potential clinical relevance of these miRNAs as minimally invasive biomarkers for ADC.
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Full list of authors on original publication

Lorraine O'Driscoll, Ken O'Byrne, John Crown, Kathy Gately, Sweta Rani

Experts in our system

1
Lorraine O'Driscoll
Trinity College Dublin
Total Publications: 164
 
2
Ken O'Byrne
Trinity College Dublin
Total Publications: 49
 
3
John Crown
Dublin City University
Total Publications: 104
 
4
Kathy Gately
Trinity College Dublin
Total Publications: 22
 
5
Sweta Rani
Trinity College Dublin