Type

Journal Article

Authors

Cliona O'Farrelly
Elizabeth J Ryan
John E. Hegarty
Justin Geoghegan
Ravichand Siddachari
Anne G Carroll
Catherine Keogh
Jean M Fletcher
Ronan Fahey
Aoife Kelly

Subjects

Biochemistry

Topics
human cells myeloid cells poly i c dendritic cells liver cells flow cytometry liver transplantation interleukin 17

CD141(+) myeloid dendritic cells are enriched in healthy human liver. (2013)

Abstract Extensive populations of liver immune cells detect and respond to homeostatic perturbation caused by damage, infection or malignancy. Dendritic cells (DCs) are central to these activities, governing the balance between tolerance and immunity. Most of our knowledge about human liver DCs is derived from studies on peritumoral tissue. Little is known about the phenotype and function of DCs, in particular the recently described CD141(+) subset, in healthy human liver and how this profile is altered in liver disease. During liver transplantation, healthy donor and diseased explant livers were perfused and hepatic mononuclear cells isolated. Dendritic cell subset frequency and phenotype were characterised in liver perfusates by flow cytometry and the function of CD141(+) DCs was evaluated by mixed lymphocyte reactions (MLRs) and measuring cytokine secretion. Almost one third of liver CD11c(+) myeloid DCs (mDCs) expressed CD141 compared to <5% of circulating mDCs. Hepatic CD141(+) DCs demonstrated pro-inflammatory function in allogeneic MLRs, inducing T cell production of interferon gamma (IFN-γ) and interleukin (IL)-17. While CD123(+) plasmacytoid DCs (pDCs) and CD1c(+) mDCs were expanded in diseased liver perfusates, CD141(+) DCs were significantly depleted. Despite their depletion, CD141(+) DCs from explant livers produced markedly increased poly(I:C)-induced IFN lambda (IFN-λ) compared with donor DCs. Accumulation of CD141(+) DCs in healthy liver, which are significantly depleted in liver disease, suggests differential involvement of mDC subsets in liver immunity.
Collections Ireland -> Trinity College Dublin -> PubMed

Full list of authors on original publication

Cliona O'Farrelly, Elizabeth J Ryan, John E. Hegarty, Justin Geoghegan, Ravichand Siddachari, Anne G Carroll, Catherine Keogh, Jean M Fletcher, Ronan Fahey, Aoife Kelly

Experts in our system

1
Cliona O'Farrelly
Trinity College Dublin
Total Publications: 147
 
2
Elizabeth J Ryan
University College Dublin
 
3
John E. Hegarty
Trinity College Dublin
Total Publications: 12
 
4
Justin Geoghegan
Trinity College Dublin
Total Publications: 14
 
5
Jean Fletcher
Trinity College Dublin
Total Publications: 25
 
6
Ronan Fahey
Trinity College Dublin
Total Publications: 5
 
7
Aoife Kelly
Trinity College Dublin
Total Publications: 5