Type

Journal Article

Authors

Yuri Volkov
Dermot Kelleher
Hardy Kornfeld
Mary P O'Sullivan
Jonathan Coleman
Philip E Lyons
Jennifer Conroy
Navin K Verma

Subjects

Biochemistry

Topics
microscopy electron transmission cell line nanocomposites silver mice autophagy biocompatible materials humans toxicity animals nanowires chemistry blotting western drug effects

Autophagy induction by silver nanowires: a new aspect in the biocompatibility assessment of nanocomposite thin films. (2012)

Abstract Nanomaterials and their enabled products have increasingly been attracting global attention due to their unique physicochemical properties. Among these emerging products, silver nanowire (AgNW)-based thin films are being developed for their promising applications in next generation nanoelectronics and nanodevices. However, serious concerns remain about possible health and safety risks they may pose. Here, we employed a multi-modal systematic biocompatibility assessment of thin films incorporating AgNW. To represent the possible routes of nanomaterial entry during occupational or environmental exposure, we employed four different cell lines of epithelial, endothelial, gastric, and phagocytic origin. Utilizing a cell-based automated image acquisition and analysis procedure in combination with real-time impedance sensing, we observed a low level of cytotoxicity of AgNW, which was dependent on cell type, nanowire lengths, doses and incubation times. Similarly, no major cytotoxic effects were induced by AgNW-containing thin films, as detected by conventional cell viability and imaging assays. However, transmission electron microscopy and Western immunoblotting analysis revealed AgNW-induced autophasosome accumulation together with an upregulation of the autophagy marker protein LC3. Autophagy represents a crucial mechanism in maintaining cellular homeostasis, and our data for the first time demonstrate triggering of such mechanism by AgNW in human phagocytic cells. Finally, atomic force microscopy revealed significant changes in the topology of cells attaching and growing on these films as substrates. Our findings thus emphasize the necessity of comprehensive biohazard assessment of nanomaterials in modern applications and devices and a thorough analysis of risks associated with their possible contact with humans through occupational or environmental exposure.
Collections Ireland -> Trinity College Dublin -> PubMed

Full list of authors on original publication

Yuri Volkov, Dermot Kelleher, Hardy Kornfeld, Mary P O'Sullivan, Jonathan Coleman, Philip E Lyons, Jennifer Conroy, Navin K Verma

Experts in our system

1
Yuri Volkov
Trinity College Dublin
Total Publications: 70
 
2
Dermot Kelleher
Trinity College Dublin
Total Publications: 149
 
3
Mary P O'Sullivan
Trinity College Dublin
Total Publications: 19
 
4
Jonathan Coleman
Trinity College Dublin
Total Publications: 217
 
5
Navin K Verma
Trinity College Dublin
Total Publications: 15