Type

Journal Article

Authors

Graham P Pidgeon
Helen M. Roche
John V Reynolds
Steven G Gray
Joanne Lysaght
Elizabeth Oliver
Emma H Allott

Subjects

Biochemistry

Topics
cells cultured gene expression cell line in vitro study enzyme activity co culture oesophageal adenocarcinoma cancer cell

The SGBS cell strain as a model for the in vitro study of obesity and cancer. (2011)

Abstract The murine adipocyte cell line 3T3-L1 is well characterised and used widely, while the human pre-adipocyte cell strain, Simpson-Golabi-Behmel Syndrome (SGBS), requires validation for use in human studies. Obesity is currently estimated to account for up to 41 % of the worldwide cancer burden. A human in vitro model system is required to elucidate the molecular mechanisms for this poorly understood association. This work investigates the relevance of the SGBS cell strain for obesity and cancer research in humans. Pre-adipocyte 3T3-L1 and SGBS were differentiated according to standard protocols. Morphology was assessed by Oil Red O staining. Adipocyte-specific gene expression was measured by qPCR and biochemical function was assessed by glycerol-3-phosphate dehydrogenase (GPDH) enzyme activity. Differential gene expression in oesophageal adenocarcinoma cell line OE33 following co-culture with SGBS or primary omental human adipocytes was investigated using Human Cancer Profiler qPCR arrays. During the process of differentiation, SGBS expressed higher levels of adipocyte-specific transcripts and fully differentiated SGBS expressed more similar morphology, transcript levels and biochemical function to primary omental adipocytes, relative to 3T3-L1. Co-culture with SGBS or primary omental adipocytes induced differential expression of genes involved in adhesion (ITGB3), angiogenesis (IGF1, TEK, TNF, VEGFA), apoptosis (GZMA, TERT) and invasion and metastasis (MMP9, TIMP3) in OE33 tumour cells. Comparable adipocyte-specific gene expression, biochemical function and a shared induced gene signature in co-cultured OE33 cells indicate that SGBS is a relevant in vitro model for obesity and cancer research in humans.
Collections Ireland -> Trinity College Dublin -> PubMed

Full list of authors on original publication

Graham P Pidgeon, Helen M. Roche, John V Reynolds, Steven G Gray, Joanne Lysaght, Elizabeth Oliver, Emma H Allott

Experts in our system

1
Graham Pidgeon
Trinity College Dublin
Total Publications: 38
 
2
Helen M. Roche
University College Dublin
Total Publications: 105
 
3
J V Reynolds
Trinity College Dublin
Total Publications: 206
 
4
Steven G Gray
Trinity College Dublin
Total Publications: 48
 
5
Joanne Lysaght
Trinity College Dublin
Total Publications: 39