Type

Journal Article

Authors

Daniela Tropea
Derek W Morris
Michael Gill
Gary Donohoe
Graham Little
Ines Molinos
Aiden P Corvin

Subjects

Biochemistry

Topics
primary cell culture biosynthesis animals newborn peptide fragments phosphorylation pharmacology cytology drug effects synapses humans recombinant proteins biological markers cerebral cortex animals mitogen activated protein kinases mice metabolism signal transduction cells cultured insulin like growth factor i neurons receptor igf type 1 phosphatidylinositol 3 kinases

Insulin-like growth factor 1 (IGF1) and its active peptide (1-3)IGF1 enhance the expression of synaptic markers in neuronal circuits through different cellular mechanisms. (2012)

Abstract Insulin-like growth factor-1 (IGF1) and its active peptide (1-3)IGF1 modulate brain growth and plasticity and are candidate molecules for treatment of brain disorders. IGF1 N-terminal portion is naturally cleaved to generate the tri-peptide (1-3)IGF1 (glycine-praline-glutamate). IGF1 and (1-3)IGF have been proposed as treatment for neuropathologies, yet their effect on nerve cells has not been directly compared. In this study we examine the effects of IGF1 and (1-3)IGF1 in primary cortical cultures and measure the expression levels of markers for intracellular pathways and synaptic function. We find that both treatments activate the IGF1 receptor and enhance the expression of synaptic markers, however, they activate different intracellular pathways. Furthermore, (1-3)IGF1 administration increases the expression of endogenous IGF1, suggesting a direct interaction between the two molecules. The results show that the two molecules increase the expression of synaptic proteins through activating different cellular mechanisms.
Collections Ireland -> Trinity College Dublin -> PubMed

Full list of authors on original publication

Daniela Tropea, Derek W Morris, Michael Gill, Gary Donohoe, Graham Little, Ines Molinos, Aiden P Corvin

Experts in our system

1
Daniela Tropea
Trinity College Dublin
Total Publications: 25
 
2
Derek Morris
Trinity College Dublin
Total Publications: 150
 
3
Michael Gill
Trinity College Dublin
Total Publications: 260
 
4
Gary Donohoe
Trinity College Dublin
Total Publications: 130
 
5
Graham Little
Trinity College Dublin
Total Publications: 7
 
6
Ines Molinos
Trinity College Dublin
Total Publications: 4
 
7
Aiden Corvin
Trinity College Dublin
Total Publications: 190