Type

Journal Article

Authors

Marek Witold Radomski
Hongzhou Zhang
Justin D Holmes
Daniel Fox
Deirdre M D'Arcy
Kamil Rahme
Carlos Medina
Iwona Inkielewicz-Stepniak
Maria Jose Santos-Martinez
Carlos Medina Martin

Subjects

Pharmacology

Topics
microscopy blood platelets physiology gold nanoparticle nanoparticles qcm quartz crystal microbalance polystyrenes quartz crystal microbalance techniques quartz crystal microbalance with dissipation scanning helium ion microscopy qcm d nanoscience materials humans metabolism nanoparticle platelet aggregation cytology flow cytometry gold polystyrene dissipation chemistry silicon dioxide p selectin light aggregometer particle size thrombocyte receptor helium padgem protein

The use of quartz crystal microbalance with dissipation (QCM-D) for studying nanoparticle-induced platelet aggregation. (2012)

Abstract Interactions between blood platelets and nanoparticles have both pharmacological and toxicological significance and may lead to platelet activation and aggregation. Platelet aggregation is usually studied using light aggregometer that neither mimics the conditions found in human microvasculature nor detects microaggregates. A new method for the measurement of platelet microaggregation under flow conditions using a commercially available quartz crystal microbalance with dissipation (QCM-D) has recently been developed. The aim of the current study was to investigate if QCM-D could be used for the measurement of nanoparticle-platelet interactions. Silica, polystyrene, and gold nanoparticles were tested. The interactions were also studied using light aggregometry and flow cytometry, which measured surface abundance of platelet receptors. Platelet activation was imaged using phase contrast and scanning helium ion microscopy. QCM-D was able to measure nanoparticle-induced platelet microaggregation for all nanoparticles tested at concentrations that were undetectable by light aggregometry and flow cytometry. Microaggregates were measured by changes in frequency and dissipation, and the presence of platelets on the sensor surface was confirmed and imaged by phase contrast and scanning helium ion microscopy.
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Full list of authors on original publication

Marek Witold Radomski, Hongzhou Zhang, Justin D Holmes, Daniel Fox, Deirdre M D'Arcy, Kamil Rahme, Carlos Medina, Iwona Inkielewicz-Stepniak, Maria Jose Santos-Martinez, Carlos Medina Martin

Experts in our system

1
Marek W Radomski
Trinity College Dublin
Total Publications: 44
 
2
Hongzhou Zhang
Trinity College Dublin
Total Publications: 50
 
3
Justin D. Holmes
University College Cork
Total Publications: 287
 
4
Daniel Fox
Trinity College Dublin
Total Publications: 15
 
5
Deirdre D'Arcy
Trinity College Dublin
Total Publications: 23
 
6
Kamil Rahme
University College Cork
Total Publications: 23
 
7
Carlos Medina
Trinity College Dublin
Total Publications: 23
 
8
Maria Jose Santos-Martinez
Trinity College Dublin
Total Publications: 29
 
9
Carlos Medina Martin
Trinity College Dublin
Total Publications: 28