Type

Journal Article

Authors

Lorraine O'Driscoll
John Crown
Marek W Radomski
Serena Germano
Claire Corcoran
Fergal C Kelleher
Keith O'Brien
Sweta Rani

Subjects

Biochemistry

Topics
cell line saliva urine ultrastructure humans micrornas proteins ascites chemistry serum genetics metabolism culture media conditioned profiling exosomes cancer microscopy electron transmission microrna cell membrane centrifugation rna messenger protein mrna gene expression profiling isolation

Isolation of exosomes for subsequent mRNA, MicroRNA, and protein profiling. (2011)

Abstract Exosomes are nano-sized, cell membrane surrounded structures that are released from many cell types. These exosomes are believed to transport a range of molecules, including mRNAs, miRNAs, and proteins; the contents depending on their cell of origin. The physiological and pathological relevance of exosomes has yet to be fully elucidated. Exosomes have been implicated in cell-to-cell communication. For example, in relation to the immune system, such exosomes may enable exchange of antigen or major histocompatibility complex-peptide complexes between antigen-bearing cells and antigen-presenting cells; in cancer, they may contain molecules that not only have relevance as biomarkers, but may also be taken up and cause adverse effects on secondary cells. Furthermore, exosomes have been proposed as autologous delivery systems that could be exploited for personalised delivery of therapeutics. In order to explore the contents and functional relevance of exosomes from medium conditioned by culture cells or from other biological fluids, prior to extensive molecular profiling, they must be isolated and purified. Here, we describe differential centrifugation methods suitable for isolating exosomes from conditioned medium and from other biological fluids, including serum, saliva, tumour ascites, and urine. We also detail Western blotting and transmission electron microscopy methods suitable for basic assessment of their presence, size, and purity, prior to progressing to global mRNA, miRNA, or protein profiling.
Collections Ireland -> Trinity College Dublin -> Pharmacy and Pharmaceutical Sciences (Scholarly Publications)
Ireland -> Trinity College Dublin -> Pharmacy and Pharmaceutical Sciences
Ireland -> Trinity College Dublin -> RSS Feeds
Ireland -> Trinity College Dublin -> PubMed
Ireland -> Trinity College Dublin -> School of Pharmacy and Pharmaceutical Sciences
Ireland -> Trinity College Dublin -> RSS Feeds

Full list of authors on original publication

Lorraine O'Driscoll, John Crown, Marek W Radomski, Serena Germano, Claire Corcoran, Fergal C Kelleher, Keith O'Brien, Sweta Rani

Experts in our system

1
Lorraine O'Driscoll
Trinity College Dublin
Total Publications: 164
 
2
John Crown
Dublin City University
Total Publications: 104
 
3
Marek W Radomski
Trinity College Dublin
Total Publications: 44
 
4
Claire Corcoran
Trinity College Dublin
 
5
Sweta Rani
Trinity College Dublin