Type

Journal Article

Authors

D A Smith
J Donegan
J Kirkham
J J Wang

Subjects

Biochemistry

Topics
carcinoma squamous cell cell line tumor humans metabolism actins microscopy atomic force high resolution

High resolution imaging of actin filaments in living cells under physilogically relevant conditions using apertureless near-field microscopy. (2010)

Abstract Living cells were used to demonstrate the potential biological applications of the apertureless SNOM when operating under fluid. An oral epithelial squamous cell carcinoma cell line (H357) was imaged under physiological-like conditions using apertureless SNOM following staining with FITC-Phalloidin which preferentially stains intracellular actin filaments of the cytoskeleton. Compared with simultaneously obtained AFM topographic image, the apertureless SNOM data provides greater detail on these cellular structures and the spatial resolution of the apertureless SNOM fluorescence image appears to be about 100 nm.
Collections Ireland -> Trinity College Dublin -> PubMed

Full list of authors on original publication

D A Smith, J Donegan, J Kirkham, J J Wang

Experts in our system

1
Diarmuid Smith
Royal College of Surgeons in Ireland
Total Publications: 34
 
2
John Francis Donegan
Trinity College Dublin
Total Publications: 152
 
3
Juan Wang
University College Dublin
Total Publications: 20