Type

Journal Article

Authors

Douwe van Sinderen
Anne L McCartney
Glenn R Gibson
Barry Hudspith
Jeremy D Sanderson
Jennifer Mahony
Jane F Turton
Knut J Heller
Horst Neve
James Murphy
and 1 others

Subjects

Microbiology

Topics
family dna replication female effluent klebsiella pneumoniae bacteriophage combination woman

Klebsiella pneumoniae subsp. pneumoniae-bacteriophage combination from the caecal effluent of a healthy woman. (2015)

Abstract A sample of caecal effluent was obtained from a female patient who had undergone a routine colonoscopic examination. Bacteria were isolated anaerobically from the sample, and screened against the remaining filtered caecal effluent in an attempt to isolate bacteriophages (phages). A lytic phage, named KLPN1, was isolated on a strain identified as Klebsiella pneumoniae subsp. pneumoniae (capsular type K2, rmpA (+)). This Siphoviridae phage presents a rosette-like tail tip and exhibits depolymerase activity, as demonstrated by the formation of plaque-surrounding haloes that increased in size over the course of incubation. When screened against a panel of clinical isolates of K. pneumoniae subsp. pneumoniae, phage KLPN1 was shown to infect and lyse capsular type K2 strains, though it did not exhibit depolymerase activity on such hosts. The genome of KLPN1 was determined to be 49,037 bp (50.53 %GC) in length, encompassing 73 predicted ORFs, of which 23 represented genes associated with structure, host recognition, packaging, DNA replication and cell lysis. On the basis of sequence analyses, phages KLPN1 (GenBank: KR262148) and 1513 (a member of the family Siphoviridae, GenBank: KP658157) were found to be two new members of the genus "Kp36likevirus."
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Douwe van Sinderen, Anne L McCartney, Glenn R Gibson, Barry Hudspith, Jeremy D Sanderson, Jennifer Mahony, Jane F Turton, Knut J Heller, Horst Neve, James Murphy and 1 others

Experts in our system

1
Jennifer Mahony
University College Cork
Total Publications: 87
 
2
Knut J Heller
University College Cork
 
3
Horst Neve
University College Cork
Total Publications: 41
 
4
James Murphy
University College Cork