Type

Journal Article

Authors

Rodolphe Barrangou
Marco Ventura
Douwe van Sinderen
Abelardo Margolles
Miguel Gueimonde
Francesca Turroni
Sabrina Duranti
Christian Milani
Gabriele Andrea Lugli
Alexandra E Briner
and 1 others

Subjects

Computer Science

Topics
acquired resistance bacteria bifidobacterium high frequency type ii co evolution adaptive immunity dna diversity endonuclease classification streptococcus thermophilus comparative genomics rna genetic engineering evolution type systems genetic tools

Occurrence and Diversity of CRISPR-Cas Systems in the Genus Bifidobacterium. (2015)

Abstract CRISPR-Cas systems constitute adaptive immune systems for antiviral defense in bacteria. We investigated the occurrence and diversity of CRISPR-Cas systems in 48 Bifidobacterium genomes to gain insights into the diversity and co-evolution of CRISPR-Cas systems within the genus and investigate CRISPR spacer content. We identified the elements necessary for the successful targeting and inference of foreign DNA in select Type II CRISPR-Cas systems, including the tracrRNA and target PAM sequence. Bifidobacterium species have a very high frequency of CRISPR-Cas occurrence (77%, 37 of 48). We found that many Bifidobacterium species have unusually large and diverse CRISPR-Cas systems that contain spacer sequences showing homology to foreign genetic elements like prophages. A large number of CRISPR spacers in bifidobacteria show perfect homology to prophage sequences harbored in the chromosomes of other species of Bifidobacterium, including some spacers that self-target the chromosome. A correlation was observed between strains that lacked CRISPR-Cas systems and the number of times prophages in that chromosome were targeted by other CRISPR spacers. The presence of prophage-targeting CRISPR spacers and prophage content may shed light on evolutionary processes and strain divergence. Finally, elements of Type II CRISPR-Cas systems, including the tracrRNA and crRNAs, set the stage for the development of genome editing and genetic engineering tools.
Collections Ireland -> University College Cork -> APC Microbiome Institute
Ireland -> University College Cork -> PubMed
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles
Ireland -> University College Cork -> Microbiology
Ireland -> University College Cork -> Research Institutes and Centres
Ireland -> University College Cork -> Microbiology - Journal Articles

Full list of authors on original publication

Rodolphe Barrangou, Marco Ventura, Douwe van Sinderen, Abelardo Margolles, Miguel Gueimonde, Francesca Turroni, Sabrina Duranti, Christian Milani, Gabriele Andrea Lugli, Alexandra E Briner and 1 others

Experts in our system

1
Marco Ventura
University College Cork
Total Publications: 31
 
2
Douwe van Sinderen
University College Cork
Total Publications: 61
 
3
Abelardo Margolles
University College Cork
 
4
Miguel Gueimonde
Teagasc
Total Publications: 6
 
5
Francesca Turroni
University College Cork
Total Publications: 8
 
6
Sabrina Duranti
University College Cork
 
7
Christian Milani
University College Cork
Total Publications: 9
 
8
Gabriele A. Lugli
University College Cork
Total Publications: 17