Type

Journal Article

Authors

Douwe van Sinderen
Christian Cambillau
Jennifer Mahony
François P. Douillard
Silvia Spinelli
Barry Collins
Stephen R Stockdale
Douwe van Sinderen

Subjects

Biochemistry

Topics
assembly crystal structure lactic acid bacteria mutagenesis gene products virion secondary structure prediction lactococcal bacteriophage tp901 1 multiple sequence alignment tail terminator protein 3 dimensional structure western blot phage lambda molecular analysis binding protein functional model lactococcus lactis protein homology detection

Structure and Assembly of TP901-1 Virion Unveiled by Mutagenesis. (2015)

Abstract Bacteriophages of the Siphoviridae family represent the most abundant viral morphology in the biosphere, yet many molecular aspects of their virion structure, assembly and associated functions remain to be unveiled. In this study, we present a comprehensive mutational and molecular analysis of the temperate Lactococcus lactis-infecting phage TP901-1. Fourteen mutations located within the structural module of TP901-1 were created; twelve mutations were designed to prevent full length translation of putative proteins by non-sense mutations, while two additional mutations caused aberrant protein production. Electron microscopy and Western blot analysis of mutant virion preparations, as well as in vitro assembly of phage mutant combinations, revealed the essential nature of many of the corresponding gene products and provided information on their biological function(s). Based on the information obtained, we propose a functional and assembly model of the TP901-1 Siphoviridae virion.
Collections Ireland -> University College Cork -> APC Microbiome Institute
Ireland -> University College Cork -> PubMed
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles
Ireland -> University College Cork -> Microbiology
Ireland -> University College Cork -> Research Institutes and Centres
Ireland -> University College Cork -> Microbiology - Journal Articles

Full list of authors on original publication

Douwe van Sinderen, Christian Cambillau, Jennifer Mahony, François P. Douillard, Silvia Spinelli, Barry Collins, Stephen R Stockdale, Douwe van Sinderen

Experts in our system

1
Christian Cambillau
University College Cork
Total Publications: 18
 
2
Jennifer Mahony
University College Cork
Total Publications: 87
 
3
Silvia Spinelli
University College Cork
Total Publications: 6
 
4
Barry Collins
University College Cork
Total Publications: 11
 
5
Stephen R. Stockdale
University College Cork
Total Publications: 15
 
6
Douwe van Sinderen
University College Cork
Total Publications: 144