Type

Journal Article

Authors

Douwe van Sinderen
Sylvain Moineau
Simon J Labrie
Denise M Tremblay
Jennifer Mahony

Subjects

Agriculture & Food Science

Topics
phage environment lactic acid bacteria groups calcium infection families dairy industry

Investigating the requirement for calcium during lactococcal phage infection. (2014)

Abstract Calcium is widely used in the study and successful propagation of virulent bacteriophages infecting lactic acid bacteria; however, it has not been assessed if and why this divalent cation is required for the infection process. Lactococcal phages are a persistent problem in the calcium-rich dairy environment and therefore were used as the model for this study. Using representative members of nine of the currently recognized ten lactococcal phage groups, encompassing phages of the Podoviridae and Siphoviridae families, we present data to suggest that calcium is not an explicit requirement for many of these phages. However, calcium expedited the pace of the lytic cycle for certain phages. Additionally, for calcium-dependent phages belonging to the 936 siphogroup, we could substitute this cation with magnesium or manganese, indicating that these phages are more adaptable than lactococcal phages of other groups. We postulate that the ability of phages to adapt to their environment and to harness the available mineral content may ultimately decide the success of a given phage infection. This may explain, in part, why 936 phages are one of the most frequently isolated phages in the dairy industry.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Douwe van Sinderen, Sylvain Moineau, Simon J Labrie, Denise M Tremblay, Jennifer Mahony

Experts in our system

1
Jennifer Mahony
University College Cork
Total Publications: 87