Type

Journal Article

Authors

Douwe van Sinderen
Marco Ventura
Lokesh Joshi
Marian Kane
Michelle Kilcoyne
Mary O'Connell Motherway
Muireann Egan
Mary O'Connell Motherway

Subjects

Microbiology

Topics
bifidobacteria phenotype dna microarray data infant human milk human colon ecosystems carbohydrate medium mammalian gastrointestinal tract intestinal tract gene expression leloir pathway sialic acid genome analysis galactose o glycosylation bifidobacterium breve ucc2003 fucose probiotic bifidobacterium breve co culture oligosaccharides cultivation anaerobic bacteria

Cross-feeding by Bifidobacterium breve UCC2003 during co-cultivation with Bifidobacterium bifidum PRL2010 in a mucin-based medium. (2014)

Abstract Bifidobacteria constitute a specific group of commensal bacteria that commonly inhabit the mammalian gastrointestinal tract. Bifidobacterium breve UCC2003 was previously shown to utilize a variety of plant/diet/host-derived carbohydrates, including cellodextrin, starch and galactan, as well as the mucin and HMO-derived monosaccharide, sialic acid. In the current study, we investigated the ability of this strain to utilize parts of a host-derived source of carbohydrate, namely the mucin glycoprotein, when grown in co-culture with the mucin-degrading Bifidobacterium bifidum PRL2010. B. breve UCC2003 was shown to exhibit growth properties in a mucin-based medium, but only when grown in the presence of B. bifidum PRL2010, which is known to metabolize mucin. A combination of HPAEC-PAD and transcriptome analyses identified some of the possible monosaccharides and oligosaccharides which support this enhanced co-cultivation growth/viability phenotype. This study describes the potential existence of a gut commensal relationship between two bifidobacterial species. We demonstrate the in vitro ability of B. breve UCC2003 to cross-feed on sugars released by the mucin-degrading activity of B. bifidum PRL2010, thus advancing our knowledge on the metabolic adaptability which allows the former strain to colonize the (infant) gut by its extensive metabolic abilities to (co-)utilize available carbohydrate sources.
Collections Ireland -> University College Cork -> APC Microbiome Institute
Ireland -> University College Cork -> PubMed
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles
Ireland -> University College Cork -> Microbiology
Ireland -> University College Cork -> Research Institutes and Centres
Ireland -> University College Cork -> Microbiology - Journal Articles

Full list of authors on original publication

Douwe van Sinderen, Marco Ventura, Lokesh Joshi, Marian Kane, Michelle Kilcoyne, Mary O'Connell Motherway, Muireann Egan, Mary O'Connell Motherway

Experts in our system

1
Douwe van Sinderen
University College Cork
 
2
Marco Ventura
University College Cork
Total Publications: 31
 
3
Lokesh Joshi
National University of Ireland Galway
Total Publications: 32
 
4
Marian Kane
Teagasc
Total Publications: 17
 
5
Michelle Kilcoyne
National University of Ireland Galway
Total Publications: 17
 
6
Mary O'Connell Motherway
University College Cork
Total Publications: 18
 
7
Muireann Egan
University College Cork
Total Publications: 6
 
8
Mary O'Connell Motherway
University College Cork
Total Publications: 16