Type

Journal Article

Authors

Douwe van Sinderen
Arjen Nauta
Mary O'Connell Motherway
Jennifer Mahony
Jochen Klumpp
Douwe van Sinderen
James Murphy
Mary O'Connell Motherway

Subjects

Agriculture & Food Science

Topics
bacteriophage resistance lactis phages bacteriophage lactococcus lactis methylome restriction modification smrt sequencing evolution bacillus subtilis phages genome sequencing genes gene expression cheese production type dna methyltransferases functional analysis single molecule methylation protection dynamic environment product quality

Methyltransferases acquired by lactococcal 936-type phage provide protection against restriction endonuclease activity. (2014)

Abstract So-called 936-type phages are among the most frequently isolated phages in dairy facilities utilising Lactococcus lactis starter cultures. Despite extensive efforts to control phage proliferation and decades of research, these phages continue to negatively impact cheese production in terms of the final product quality and consequently, monetary return. Whole genome sequencing and in silico analysis of three 936-type phage genomes identified several putative (orphan) methyltransferase (MTase)-encoding genes located within the packaging and replication regions of the genome. Utilising SMRT sequencing, methylome analysis was performed on all three phages, allowing the identification of adenine modifications consistent with N-6 methyladenine sequence methylation, which in some cases could be attributed to these phage-encoded MTases. Heterologous gene expression revealed that M.Phi145I/M.Phi93I and M.Phi93DAM, encoded by genes located within the packaging module, provide protection against the restriction enzymes HphI and DpnII, respectively, representing the first functional MTases identified in members of 936-type phages. SMRT sequencing technology enabled the identification of the target motifs of MTases encoded by the genomes of three lytic 936-type phages and these MTases represent the first functional MTases identified in this species of phage. The presence of these MTase-encoding genes on 936-type phage genomes is assumed to represent an adaptive response to circumvent host encoded restriction-modification systems thereby increasing the fitness of the phages in a dynamic dairy environment.
Collections Ireland -> University College Cork -> APC Microbiome Institute
Ireland -> University College Cork -> PubMed
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles
Ireland -> University College Cork -> Microbiology
Ireland -> University College Cork -> Research Institutes and Centres
Ireland -> University College Cork -> Microbiology - Journal Articles

Full list of authors on original publication

Douwe van Sinderen, Arjen Nauta, Mary O'Connell Motherway, Jennifer Mahony, Jochen Klumpp, Douwe van Sinderen, James Murphy, Mary O'Connell Motherway

Experts in our system

1
Arjen Nauta
University College Cork
Total Publications: 11
 
2
Jennifer Mahony
University College Cork
Total Publications: 87
 
3
Douwe van Sinderen
University College Cork
Total Publications: 144
 
4
James Murphy
University College Cork
 
5
Mary O'Connell Motherway
University College Cork