Type

Journal Article

Authors

Colin Hill
Julian R Marchesi
Roy D. Sleator
Eamonn P Culligan

Subjects

Biochemistry

Topics
gut staphylococcus aureus stress response binding protein family cell membrane sequence alignments oxidative stress membrane protein salt tolerance bacterial stress escherichia coli listeria monocytogenes human gastrointestinal tract gene sigma factor

Metagenomic identification of a novel salt tolerance gene from the human gut microbiome which encodes a membrane protein with homology to a brp/blh-family β-carotene 15,15'-monooxygenase. (2014)

Abstract The human gut microbiome consists of at least 3 million non-redundant genes, 150 times that of the core human genome. Herein, we report the identification and characterisation of a novel stress tolerance gene from the human gut metagenome. The locus, assigned brpA, encodes a membrane protein with homology to a brp/blh-family β-carotene monooxygenase. Cloning and heterologous expression of brpA in Escherichia coli confers a significant salt tolerance phenotype. Furthermore, when cultured in the presence of exogenous β-carotene, cell pellets adopt a red/orange pigmentation indicating the incorporation of carotenoids in the cell membrane.
Collections Ireland -> University College Cork -> APC Microbiome Institute
Ireland -> University College Cork -> PubMed
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles
Ireland -> University College Cork -> Microbiology
Ireland -> University College Cork -> Research Institutes and Centres
Ireland -> University College Cork -> Microbiology - Journal Articles

Full list of authors on original publication

Colin Hill, Julian R Marchesi, Roy D. Sleator, Eamonn P Culligan

Experts in our system

1
Colin Hill
University College Cork
Total Publications: 351
 
2
Julian R Marchesi
University College Cork
Total Publications: 40
 
3
Roy D. Sleator
University College Cork
Total Publications: 44
 
4
Eamonn P Culligan
University College Cork
Total Publications: 13