Type

Journal Article

Authors

Douwe van Sinderen
Fabio Dal Bello
Jean-Paul Noben
Knut J Heller
Horst Neve
Anneleen Cornelissen
Francesca Bottacini
Mary O'Connell Motherway
Jennifer Mahony
Eoghan Casey

Subjects

Chemistry

Topics
lactobacillus molecular phages industrial fermentations biological mass spectrometry physical genetic

Molecular characterization of three Lactobacillus delbrueckii subsp. bulgaricus phages. (2014)

Abstract In this study, three phages infecting Lactobacillus delbrueckii subsp. bulgaricus, named Ld3, Ld17, and Ld25A, were isolated from whey samples obtained from various industrial fermentations. These phages were further characterized in a multifaceted approach: (i) biological and physical characterization through host range analysis and electron microscopy; (ii) genetic assessment through genome analysis; (iii) mass spectrometry analysis of the structural components of the phages; and (iv), for one phage, transcriptional analysis by Northern hybridization, reverse transcription-PCR, and primer extension. The three obtained phage genomes display high levels of sequence identity to each other and to genomes of the so-called group b L. delbrueckii phages c5, LL-Ku, and phiLdb, where some of the observed differences are believed to be responsible for host range variations.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Douwe van Sinderen, Fabio Dal Bello, Jean-Paul Noben, Knut J Heller, Horst Neve, Anneleen Cornelissen, Francesca Bottacini, Mary O'Connell Motherway, Jennifer Mahony, Eoghan Casey

Experts in our system

1
Fabio Dal Bello
University College Cork
Total Publications: 7
 
2
Jean-Paul Noben
University College Cork
Total Publications: 13
 
3
Knut J Heller
University College Cork
 
4
Horst Neve
University College Cork
Total Publications: 41
 
5
Francesca Bottacini
University College Cork
Total Publications: 32
 
6
Jennifer Mahony
University College Cork
Total Publications: 87
 
7
Eoghan Casey
University College Cork
Total Publications: 9