Type

Journal Article

Authors

Douwe van Sinderen
Jennifer Mahony
Stuart Ainsworth

Subjects

Microbiology

Topics
virology classification bacteriophages phenotype lactococcus lactis growth development dna bacterial chromosomes bacterial computational biology plasmids isolation purification cloning molecular genetics

The plasmid complement of Lactococcus lactis UC509.9 encodes multiple bacteriophage resistance systems. (2014)

Abstract Lactococcus lactis subsp. cremoris strains are used globally for the production of fermented dairy products, particularly hard cheeses. Believed to be of plant origin, L. lactis strains that are used as starter cultures have undergone extensive adaptation to the dairy environment, partially through the acquisition of extrachromosomal DNA in the form of plasmids that specify technologically important phenotypic traits. Here, we present a detailed analysis of the eight plasmids of L. lactis UC509.9, an Irish dairy starter strain. Key industrial phenotypes were mapped, and genes that are typically associated with lactococcal plasmids were identified. Four distinct, plasmid-borne bacteriophage resistance systems were identified, including two abortive infection systems, AbiB and AbiD1, thereby supporting the observed phage resistance of L. lactis UC509.9. AbiB escape mutants were generated for phage sk1, which were found to carry mutations in orf6, which encodes the major capsid protein of this phage.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Douwe van Sinderen, Jennifer Mahony, Stuart Ainsworth

Experts in our system

1
Jennifer Mahony
University College Cork
Total Publications: 87
 
2
Stuart Ainsworth
University College Cork