Type

Journal Article

Authors

Colin Hill
R Paul Ross
Paula O'Connor
Kiera Considine
Thérèse Deasy
Tanya Clifford
Maire Begley
Avelino Alvarez-Ordóñez

Subjects

Microbiology

Topics
lactococcus lactis matrix assisted laser bacillus licheniformis escherichia coli listeria monocytogenes staphylococcus aureus time of flight mass spectrometry antimicrobial activity

Investigation of the Antimicrobial Activity of Bacillus licheniformis Strains Isolated from Retail Powdered Infant Milk Formulae. (2014)

Abstract This study investigated the potential antimicrobial activity of ten Bacillus licheniformis strains isolated from retail infant milk formulae against a range of indicator (Lactococcus lactis, Lactobacillus bulgaricus and Listeria innocua) and clinically relevant (Listeria monocytogenes, Staphylococcus aureus, Streptococcus agalactiae, Salmonella Typhimurium and Escherichia coli) microorganisms. Deferred antagonism assays confirmed that all B. licheniformis isolates show antimicrobial activity against the Gram-positive target organisms. PCR and matrix-assisted laser desorption ionization time-of-flight mass spectrometry analyses indicated that four of the B. licheniformis isolates produce the bacteriocin lichenicidin. The remaining six isolates demonstrated a higher antimicrobial potency than lichenicidin-producing strains. Further analyses identified a peptide of ~1,422 Da as the most likely bioactive responsible for the antibacterial activity of these six isolates. N-terminal sequencing of the ~1,422 Da peptide from one strain identified it as ILPEITXIFHD. This peptide shows a high homology to the non-ribosomal peptides bacitracin and subpeptin, known to be produced by Bacillus spp. Subsequent PCR analyses demonstrated that the six B. licheniformis isolates may harbor the genetic machinery needed for the synthesis of a non-ribosomal peptide synthetase similar to those involved in production of subpeptin and bacitracin, which suggests that the ~1,422 Da peptide might be a variant of subpeptin and bacitracin.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Colin Hill, R Paul Ross, Paula O'Connor, Kiera Considine, Thérèse Deasy, Tanya Clifford, Maire Begley, Avelino Alvarez-Ordóñez

Experts in our system

1
Colin Hill
University College Cork
Total Publications: 351
 
2
R Paul Ross
Teagasc
Total Publications: 441
 
3
Paula M. O'Connor
Teagasc
Total Publications: 85
 
4
Kiera Considine
University College Cork
 
5
Thérèse Deasy
University College Cork
Total Publications: 4
 
6
Tanya Clifford
University College Cork
Total Publications: 4
 
7
Maire Begley
University College Cork
Total Publications: 27
 
8
Avelino Alvarez-Ordóñez
University College Cork
Total Publications: 6