Type

Journal Article

Authors

Colin Hill
Julian R Marchesi
Roy D. Sleator
Eamonn P Culligan

Subjects

Microbiology

Topics
ankyrin repeat intestines microbiota genes bacterial akkermansia muciniphila datasets as topic structure prediction male adult osmotic stress benzodiazepine receptor i tasser outer membrane protein salt tolerance bacterial proteins humans sequence analysis escherichia coli genetic loci metagenomics gastrointestinal tract microbiology genetics

Functional environmental screening of a metagenomic library identifies stlA; a unique salt tolerance locus from the human gut microbiome. (2013)

Abstract Functional environmental screening of metagenomic libraries is a powerful means to identify and assign function to novel genes and their encoded proteins without any prior sequence knowledge. In the current study we describe the identification and subsequent analysis of a salt-tolerant clone from a human gut metagenomic library. Following transposon mutagenesis we identified an unknown gene (stlA, for "salt tolerance locus A") with no current known homologues in the databases. Subsequent cloning and expression in Escherichia coli MKH13 revealed that stlA confers a salt tolerance phenotype in its surrogate host. Furthermore, a detailed in silico analysis was also conducted to gain additional information on the properties of the encoded StlA protein. The stlA gene is rare when searched against human metagenome datasets such as MetaHit and the Human Microbiome Project and represents a novel and unique salt tolerance determinant which appears to be found exclusively in the human gut environment.
Collections Ireland -> University College Cork -> APC Microbiome Institute
Ireland -> University College Cork -> PubMed
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles
Ireland -> University College Cork -> Microbiology
Ireland -> University College Cork -> Research Institutes and Centres
Ireland -> University College Cork -> Microbiology - Journal Articles

Full list of authors on original publication

Colin Hill, Julian R Marchesi, Roy D. Sleator, Eamonn P Culligan

Experts in our system

1
Colin Hill
University College Cork
Total Publications: 351
 
2
Julian R Marchesi
University College Cork
Total Publications: 40
 
3
Roy D. Sleator
University College Cork
Total Publications: 44
 
4
Eamonn P Culligan
University College Cork
Total Publications: 13