Type

Journal Article

Authors

Douwe van Sinderen
Arjen Nauta
Stuart Ainsworth
Jennifer Mahony
James Murphy

Subjects

Microbiology

Topics
evolution molecular genetics metabolism bacteria enzymology bacteriophages dna methylation methyltransferases

Bacteriophage orphan DNA methyltransferases: insights from their bacterial origin, function, and occurrence. (2013)

Abstract Type II DNA methyltransferases (MTases) are enzymes found ubiquitously in the prokaryotic world, where they play important roles in several cellular processes, such as host protection and epigenetic regulation. Three classes of type II MTases have been identified thus far in bacteria which function in transferring a methyl group from S-adenosyl-l-methionine (SAM) to a target nucleotide base, forming N-6-methyladenine (class I), N-4-methylcytosine (class II), or C-5-methylcytosine (class III). Often, these MTases are associated with a cognate restriction endonuclease (REase) to form a restriction-modification (R-M) system protecting bacterial cells from invasion by foreign DNA. When MTases exist alone, which are then termed orphan MTases, they are believed to be mainly involved in regulatory activities in the bacterial cell. Genomes of various lytic and lysogenic phages have been shown to encode multi- and mono-specific orphan MTases that have the ability to confer protection from restriction endonucleases of their bacterial host(s). The ability of a phage to overcome R-M and other phage-targeting resistance systems can be detrimental to particular biotechnological processes such as dairy fermentations. Conversely, as phages may also be beneficial in certain areas such as phage therapy, phages with additional resistance to host defenses may prolong the effectiveness of the therapy. This minireview will focus on bacteriophage-encoded MTases, their prevalence and diversity, as well as their potential origin and function.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Douwe van Sinderen, Arjen Nauta, Stuart Ainsworth, Jennifer Mahony, James Murphy

Experts in our system

1
Arjen Nauta
University College Cork
Total Publications: 11
 
2
Stuart Ainsworth
University College Cork
 
3
Jennifer Mahony
University College Cork
Total Publications: 87
 
4
James Murphy
University College Cork