Type

Journal Article

Authors

Colin Hill
Maire Begley
Claire Flaherty

Subjects

Veterinary

Topics
infant formula integration infant milk strains real time cronobacter cocktail

Investigation of the use of a cocktail of lux-tagged cronobacter strains for monitoring growth in infant milk formulae. (2013)

Abstract The objective of the present study was to create a collection of Lux-tagged Cronobacter strains to determine whether bioluminescence could be used to monitor growth of this pathogen in infant milk formula (IMF). Nine Cronobacter strains (seven C. sakazakii, one C. malonaticus, and one C. muytjensii) were transformed with plasmid p16S lux, and integration of the plasmid at the desired site on the chromosome was confirmed by PCR. The integrated plasmid was stable in the absence of antibiotic selection, and growth of the Lux-tagged strains was similar to that of their nontagged counterparts. Growth of Lux-tagged strains was monitored in real time in 10 commercial brands of IMF by measuring light emission with a luminometer. Although all of the IMF samples tested were able to support the growth of the Cronobacter strains, differences were observed among IMF brands. Variations in the amount of light emitted by individual Cronobacter strains were also noted. Monitoring light emission with a combination of two strains that produced higher and lower than average relative light readings was a good surrogate for evaluating the entire collection of Lux-tagged strains.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Colin Hill, Maire Begley, Claire Flaherty

Experts in our system

1
Colin Hill
University College Cork
Total Publications: 351
 
2
Maire Begley
University College Cork
Total Publications: 27