Type

Journal Article

Authors

Douwe van Sinderen
Arjen Nauta
Martin Bonestroo
Horst Neve
Knut Heller
Lesley Hoyles
Jennifer Mahony
Bridget Royer
James Murphy

Subjects

Agriculture & Food Science

Topics
bacteriophages type lactococcus lactis cheese plants isolated cheese production biodiversity

Biodiversity of lactococcal bacteriophages isolated from 3 Gouda-type cheese-producing plants. (2013)

Abstract This study reports on the identification and characterization of bacteriophages isolated from cheese-production facilities that use undefined, mixed starter cultures. Phage screening was carried out on whey samples isolated from 3 factories, 2 utilizing one particular undefined starter mixture and 1 utilizing another undefined starter mixture. Phage screening was carried out using 40 strains isolated from the 2 mixed starter cultures, and phages were profiled using host range, electron microscopy, multiplex PCR, and DNA restriction analysis. Twenty distinct lactococcal phages were identified based on host range and DNA restriction profiles, all belonging to the 936-type phage species. Nineteen of these phages were found to be able to infect both recognized subspecies of Lactococcus lactis. Restriction of phage DNA isolated using a newly developed guanidinium thiocyanate disruption method showed that the genomes of the 20 isolated phages were between 26 and 31kb in size. It is evident from this study that the use of mixed starters creates an ideal environment for the proliferation of different phages with slightly varying host ranges. Furthermore, in this environment, members of the 936-type phage species clearly dominated the phage population.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Douwe van Sinderen, Arjen Nauta, Martin Bonestroo, Horst Neve, Knut Heller, Lesley Hoyles, Jennifer Mahony, Bridget Royer, James Murphy

Experts in our system

1
Arjen Nauta
University College Cork
Total Publications: 11
 
2
Horst Neve
University College Cork
Total Publications: 41
 
3
Knut J Heller
University College Cork
 
4
Jennifer Mahony
University College Cork
Total Publications: 87
 
5
James Murphy
University College Cork