Type

Journal Article

Authors

Douwe van Sinderen
Finn K Vogensen
Christian Cambillau
Karin Hammer
Søren J Sørensen
Knut J Heller
Lars H Hansen
Horst Neve
Stuart Ainsworth
James Murphy
and 2 others

Subjects

Biochemistry

Topics
phylogeny protein binding comparative genomics lactococcus lactis cell wall genotype binding proteins receptor

Investigation of the relationship between lactococcal host cell wall polysaccharide genotype and 936 phage receptor binding protein phylogeny. (2013)

Abstract Comparative genomics of 11 lactococcal 936-type phages combined with host range analysis allowed subgrouping of these phage genomes, particularly with respect to their encoded receptor binding proteins. The so-called pellicle or cell wall polysaccharide of Lactococcus lactis, which has been implicated as a host receptor of (certain) 936-type phages, is specified by a large gene cluster, which, among different lactococcal strains, contains highly conserved regions as well as regions of diversity. The regions of diversity within this cluster on the genomes of lactococcal strains MG1363, SK11, IL1403, KF147, CV56, and UC509.9 were used for the development of a multiplex PCR system to identify the pellicle genotype of lactococcal strains used in this study. The resulting comparative analysis revealed an apparent correlation between the pellicle genotype of a given host strain and the host range of tested 936-type phages. Such a correlation would allow prediction of the intrinsic 936-type phage sensitivity of a particular lactococcal strain and substantiates the notion that the lactococcal pellicle polysaccharide represents the receptor for (certain) 936-type phages while also partially explaining the molecular reasons behind the observed narrow host range of such phages.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Douwe van Sinderen, Finn K Vogensen, Christian Cambillau, Karin Hammer, Søren J Sørensen, Knut J Heller, Lars H Hansen, Horst Neve, Stuart Ainsworth, James Murphy and 2 others

Experts in our system

1
Christian Cambillau
University College Cork
Total Publications: 18
 
2
Knut J Heller
University College Cork
 
3
Horst Neve
University College Cork
Total Publications: 41
 
4
Stuart Ainsworth
University College Cork
 
5
James Murphy
University College Cork