Type

Journal Article

Authors

Douwe van Sinderen
Sylvain Moineau
Knut J Heller
Horst Neve
Denise M Tremblay
Bruno Martel
Jennifer Mahony

Subjects

Biology

Topics
phages dna replication species electron north america molecular analysis groups cell

Identification of a new P335 subgroup through molecular analysis of lactococcal phages Q33 and BM13. (2013)

Abstract Lactococcal dairy starter strains are under constant threat from phages in dairy fermentation facilities, especially by members of the so-called 936, P335, and c2 species. Among these three phage groups, members of the P335 species are the most genetically diverse. Here, we present the complete genome sequences of two P335-type phages, Q33 and BM13, isolated in North America and representing a novel lineage within this phage group. The Q33 and BM13 genomes exhibit homology, not only to P335-type, but also to elements of the 936-type phage sequences. The two phage genomes also have close relatedness to phages infecting Enterococcus and Clostridium, a heretofore unknown feature among lactococcal P335 phages. The Q33 and BM13 genomes are organized in functionally related clusters with genes encoding functions such as DNA replication and packaging, morphogenesis, and host cell lysis. Electron micrographic analysis of the two phages highlights the presence of a baseplate more reminiscent of the baseplate of 936 phages than that of the majority of members of the P335 group, with the exception of r1t and LC3.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Douwe van Sinderen, Sylvain Moineau, Knut J Heller, Horst Neve, Denise M Tremblay, Bruno Martel, Jennifer Mahony

Experts in our system

1
Knut J Heller
University College Cork
 
2
Horst Neve
University College Cork
Total Publications: 41
 
3
Jennifer Mahony
University College Cork
Total Publications: 87