Type

Journal Article

Authors

Yrjö H Roos
Joseph P Kerry
Naritchaya Potes

Subjects

Chemistry

Topics
whey protein water oxidation reaction water activity protein aggregation protein denaturation oil denaturation and aggregation

Oil as reaction medium for glycation, oxidation, denaturation, and aggregation of whey protein systems of low water activity. (2013)

Abstract Whey protein isolate (WPI)-oil (75:25) and WPI-oil-(glucose-fructose) (45:15:40) as models of high-protein systems containing either olive (OO) or sunflower oil (SO) were stored at 20 or 40 °C to investigate component interactions. The indicators of protein oxidation (carbonyl content) and aggregation (total sulfhydryl content) and heats of protein denaturation and aggregation were investigated. Highest levels of disulfide bonding and carbonyls in WPI-OO formed during the first 2 weeks of storage concomitantly with enhanced protein aggregation. WPI-OO and WPI-SO systems (prestorage) showed increased protein denaturation temperature. The WPI proteins showed higher heat sensitivity with OO or SO at 40 °C, and the system with OO showed preaggregated protein as found from decreased heats of protein aggregation. OO or SO in WPI-oil-(glucose-fructose) systems reduced heats of protein aggregation. Lipid oxidation products and nonenzymatic browning reactions in glucose-fructose-containing systems decreased the solubility of solids and increased protein aggregation, hydrophobicity, and hardening of structure.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Yrjö H Roos, Joseph P Kerry, Naritchaya Potes

Experts in our system

1
Yrjö H Roos
University College Cork
Total Publications: 27
 
2
J P Kerry
University College Cork
Total Publications: 130
 
3
Naritchaya Potes
University College Cork
Total Publications: 4