Type

Journal Article

Authors

Roy D. Sleator
Colin Hill
Audrey Feeney
Susan McLernon
Roland F Hoffmann

Subjects

Microbiology

Topics
tolerance single stress listeria monocytogenes transport systems e coli point mutation promoter

A single point mutation in the listerial betL σ (A) -dependent promoter leads to improved osmo- and chill-tolerance and a morphological shift at elevated osmolarity. (2013)

Abstract Betaine uptake in Listeria monocytogenes is mediated by three independent transport systems, the simplest of which in genetic terms is the secondary transporter BetL. Using a random mutagenesis approach, based on the E. coli XL1 Red mutator strain, we identified a single point mutation in a putative promoter region upstream of the BetL coding region which leads to a significant increase in betL transcript levels under osmo- and chill-stress conditions and a concomitant increase in stress tolerance. Furthermore, the mutation appears to counter the heretofore unreported "twisted" cell morphology observed for L. monocytogenes grown at elevated osmolarities in tryptone soy broth.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Roy D. Sleator, Colin Hill, Audrey Feeney, Susan McLernon, Roland F Hoffmann

Experts in our system

1
Roy D. Sleator
University College Cork
Total Publications: 44
 
2
Colin Hill
University College Cork
Total Publications: 351